Detailed information of TA system TA024515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4566106..4566737
• Replicon: chromosome
• Accession: NC_013716
• Organism: Citrobacter rodentium ICC168

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D2TKP7
• Locus tag: ROD_RS21275
• Protein ID: WP_012908247.1
• Coordinates: 4566106..4566381 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: D2TKP8
• Locus tag: ROD_RS21280
• Protein ID: WP_012908248.1
• Coordinates: 4566378..4566737 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ROD_RS21255	4561340..4562503	-	1164	WP_012908243.1	metallo-dependent hydrolase	-
ROD_RS21260	4562516..4563910	-	1395	WP_012908244.1	DASS family sodium-coupled anion symporter	-
ROD_RS21265	4563976..4564674	-	699	WP_012908245.1	RraA family protein	-
ROD_RS21270	4564685..4565638	-	954	WP_012908246.1	hydroxyacid dehydrogenase	-
ROD_RS21275	4566106..4566381	+	276	WP_012908247.1	type II toxin-antitoxin system HicA family toxin	Toxin
ROD_RS21280	4566378..4566737	+	360	WP_012908248.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ROD_RS28450	4567030..4567431	-	402	WP_024133026.1	nickel-responsive transcriptional regulator NikR	-
ROD_RS21290	4567444..4568244	-	801	WP_012908250.1	nickel import ATP-binding protein NikE	-
ROD_RS21295	4568241..4569005	-	765	WP_012908251.1	nickel import ATP-binding protein NikD	-
ROD_RS21300	4569005..4569838	-	834	WP_012908252.1	nickel ABC transporter permease subunit NikC	-
ROD_RS21305	4569835..4570779	-	945	WP_012908253.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10265.00 Da
• Isoelectric Point: 10.8675

>T24515 WP_012908247.1 NC_013716:4566106-4566381 [Citrobacter rodentium ICC168]
MRQQVLSLRKKQRNTLEQLFKTPVPQGIKWTEIESLVKALGGVIKEGRGSRCKFLLNNSIACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T24515 NC_013716:4566106-4566381 [Citrobacter rodentium ICC168]
ATGAGACAGCAGGTGTTATCCTTACGAAAAAAGCAAAGAAATACGCTGGAACAGCTCTTTAAAACACCTGTACCACAAGG
AATAAAGTGGACAGAGATAGAATCCCTGGTGAAGGCGTTGGGCGGCGTAATTAAGGAAGGGCGCGGCTCGCGCTGCAAAT
TCTTATTGAACAACAGTATTGCCTGTTTTCACCGTCCTCATCCTTCACCCGATACGGATAAAGGCGCAGTGGAGAGTGTG
CGTGACTGGCTACTCAGCATAGGGGTTAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13276.01 Da
• Isoelectric Point: 4.7612

>AT24515 WP_012908248.1 NC_013716:4566378-4566737 [Citrobacter rodentium ICC168]
MIKPKTPNAMEIAGHPAVINYVPELNAFRGKFLGLSGHCDFVSDSIQGLQEEGEISLNEYLEDCREAGIEPYAHAEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWIIDTLSKRLKQA

Nucleotide

• Length: 360 bp

>AT24515 NC_013716:4566378-4566737 [Citrobacter rodentium ICC168]
ATGATTAAACCTAAAACGCCAAATGCAATGGAAATCGCGGGGCACCCTGCGGTAATCAATTATGTGCCTGAGCTAAACGC
GTTTCGCGGTAAGTTTCTTGGGCTATCAGGACATTGCGATTTTGTCTCGGATAGCATCCAGGGCTTGCAGGAGGAGGGCG
AGATCTCTTTAAACGAATACCTTGAAGATTGCCGGGAGGCGGGTATTGAACCTTATGCGCATGCGGAAAAAATGAAAACA
TTTACGCTTCGCTATCCGGAGTCATTTGGCGAACGTTTAAGTTCCGCGGCGGCAGAAGAACAGGTTTCAGTGAATACCTG
GATCATTGATACGCTTAGCAAACGTCTGAAGCAGGCTTAG

Structures

Toxin

• AlphaFold DB: D2TKP7

Antitoxin

• AlphaFold DB: D2TKP8