Detailed information of TA system TA024509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 3467114..3467709
• Replicon: chromosome
• Accession: NC_013716
• Organism: Citrobacter rodentium ICC168

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: D2TN60
• Locus tag: ROD_RS16205
• Protein ID: WP_012907372.1
• Coordinates: 3467359..3467709 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: D2TN59
• Locus tag: ROD_RS16200
• Protein ID: WP_012907371.1
• Coordinates: 3467114..3467365 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ROD_RS16190	3462780..3466559	+	3780	WP_012907369.1	autotransporter assembly complex protein TamB	-
ROD_RS16195	3466562..3466906	+	345	WP_012907370.1	gamma-glutamylcyclotransferase	-
ROD_RS16200	3467114..3467365	+	252	WP_012907371.1	type II toxin-antitoxin system ChpS family antitoxin	Antitoxin
ROD_RS16205	3467359..3467709	+	351	WP_012907372.1	type II toxin-antitoxin system ChpB family toxin	Toxin
ROD_RS16210	3467808..3468338	-	531	WP_012907373.1	inorganic diphosphatase	-
ROD_RS16215	3468646..3469602	+	957	WP_012907374.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
ROD_RS16220	3469759..3471261	+	1503	WP_012907375.1	sugar ABC transporter ATP-binding protein	-
ROD_RS16225	3471275..3472297	+	1023	WP_012907376.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-115)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12789.83 Da
• Isoelectric Point: 5.1582

>T24509 WP_012907372.1 NC_013716:3467359-3467709 [Citrobacter rodentium ICC168]
MVKEQVFERGDIVLVGFEPASGHEQQGKRRPALVLSVRPFNQMGMTLVAPITQGGNYARYAGFCVPLHCEEGDIQGMVLI
NQIRMLDLRAGEARRIGLAADEVVEDALLRLQAIVE

Nucleotide

• Length: 351 bp

>T24509 NC_013716:3467359-3467709 [Citrobacter rodentium ICC168]
ATGGTGAAGGAGCAGGTATTCGAACGGGGAGACATTGTGCTGGTCGGCTTCGAGCCGGCAAGCGGTCATGAACAACAGGG
TAAGCGTCGGCCTGCGCTGGTTCTCTCCGTTCGTCCATTTAATCAGATGGGGATGACTCTGGTTGCGCCCATCACCCAGG
GGGGGAACTATGCGCGTTACGCCGGGTTTTGCGTTCCGCTTCACTGTGAGGAGGGAGATATTCAGGGAATGGTGCTGATC
AACCAGATACGGATGCTGGATCTCCGCGCCGGAGAGGCCCGTCGCATAGGCCTGGCTGCGGATGAGGTGGTCGAAGATGC
GTTATTAAGATTGCAGGCCATCGTGGAATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9227.62 Da
• Isoelectric Point: 4.4963

>AT24509 WP_012907371.1 NC_013716:3467114-3467365 [Citrobacter rodentium ICC168]
MRITIKKWGNSAGMVIPNVVMKELGLQPGQSMEAHVTNNQLVLTPAIKRYSLEELLAQCDPQAPEIREQEIWGAADPAGD
EIW

Nucleotide

• Length: 252 bp

>AT24509 NC_013716:3467114-3467365 [Citrobacter rodentium ICC168]
ATGCGAATTACCATTAAGAAATGGGGAAACAGTGCCGGTATGGTGATACCAAACGTGGTGATGAAAGAGCTTGGCTTACA
GCCGGGACAGAGTATGGAGGCCCATGTGACGAACAATCAGTTAGTGCTGACGCCCGCCATAAAGCGGTATTCGCTGGAGG
AACTGCTTGCGCAGTGCGATCCGCAGGCGCCTGAAATACGCGAACAGGAGATATGGGGAGCCGCGGATCCGGCTGGTGAT
GAAATATGGTGA

Structures

Toxin

• AlphaFold DB: D2TN60

Antitoxin

• AlphaFold DB: D2TN59