Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1458761..1459571 Replicon chromosome
Accession NZ_CP097248
Organism Klebsiella pneumoniae strain NTU107224

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag M5D84_RS07100 Protein ID WP_004178461.1
Coordinates 1458761..1459294 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag M5D84_RS07105 Protein ID WP_002887278.1
Coordinates 1459305..1459571 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5D84_RS07095 (1457592) 1457592..1458713 + 1122 WP_040188022.1 cupin domain-containing protein -
M5D84_RS07100 (1458761) 1458761..1459294 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
M5D84_RS07105 (1459305) 1459305..1459571 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
M5D84_RS07110 (1459674) 1459674..1461107 - 1434 WP_065782632.1 Cu(+)/Ag(+) sensor histidine kinase -
M5D84_RS07115 (1461097) 1461097..1461780 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
M5D84_RS07120 (1461953) 1461953..1463338 + 1386 WP_065782630.1 efflux transporter outer membrane subunit -
M5D84_RS07125 (1463356) 1463356..1463700 + 345 WP_065519257.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T245022 WP_004178461.1 NZ_CP097248:c1459294-1458761 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT245022 WP_002887278.1 NZ_CP097248:c1459571-1459305 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References