Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2865069..2865697 Replicon chromosome
Accession NZ_CP097245
Organism Leptospira santarosai strain DCP-017

Toxin (Protein)


Gene name vapC Uniprot ID K8Y570
Locus tag M5D10_RS13035 Protein ID WP_004463182.1
Coordinates 2865069..2865467 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID M6ZBJ3
Locus tag M5D10_RS13040 Protein ID WP_004468228.1
Coordinates 2865467..2865697 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5D10_RS13005 (M5D10_12980) 2860244..2860633 - 390 WP_004480843.1 helix-turn-helix transcriptional regulator -
M5D10_RS13010 (M5D10_12985) 2860958..2862079 + 1122 WP_269025368.1 helix-turn-helix domain-containing protein -
M5D10_RS13015 (M5D10_12990) 2862082..2862258 - 177 WP_269025369.1 hypothetical protein -
M5D10_RS13020 (M5D10_12995) 2862319..2863752 + 1434 WP_269026461.1 DDE-type integrase/transposase/recombinase -
M5D10_RS13025 (M5D10_13000) 2864097..2864378 + 282 WP_032927939.1 type II toxin-antitoxin system RelE/ParE family toxin -
M5D10_RS13030 (M5D10_13005) 2864394..2864690 + 297 WP_016757297.1 HigA family addiction module antitoxin -
M5D10_RS13035 (M5D10_13010) 2865069..2865467 - 399 WP_004463182.1 type II toxin-antitoxin system VapC family toxin Toxin
M5D10_RS13040 (M5D10_13015) 2865467..2865697 - 231 WP_004468228.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
M5D10_RS13045 (M5D10_13020) 2865924..2866136 - 213 WP_269025370.1 hypothetical protein -
M5D10_RS13050 (M5D10_13025) 2866185..2866358 - 174 WP_004468364.1 hypothetical protein -
M5D10_RS13055 (M5D10_13030) 2866403..2869147 - 2745 WP_269025371.1 isoleucine--tRNA ligase -
M5D10_RS13060 (M5D10_13035) 2869155..2869808 - 654 WP_004468285.1 leucine-rich repeat domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14980.51 Da        Isoelectric Point: 9.4739

>T245018 WP_004463182.1 NZ_CP097245:c2865467-2865069 [Leptospira santarosai]
MYLLDTNICIFLIKKKSQKLLDKLKKNFNKGLYISSLTLAELEFGIENSEQKEKNRVSLIEFLSIFEILPFEQSDAQAFG
ILKADLKKSGNPIGSIDALLAAQALSRVLVLITNNTKEFKRVKNLSIEDWSQ

Download         Length: 399 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8783.07 Da        Isoelectric Point: 8.7070

>AT245018 WP_004468228.1 NZ_CP097245:c2865697-2865467 [Leptospira santarosai]
MHTAKLFINGRSQAVRLPKEFQFKGDDVFIQKIGEAVILVPKNKAWNVFLDGLNGFTEDFLKEGREGRPETEREKF

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2R4L8Y5

Antitoxin

Source ID Structure
AlphaFold DB M6ZBJ3

References