Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 2692866..2693442 Replicon chromosome
Accession NZ_CP097245
Organism Leptospira santarosai strain DCP-017

Toxin (Protein)


Gene name chpK Uniprot ID -
Locus tag M5D10_RS12265 Protein ID WP_269025250.1
Coordinates 2692866..2693207 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID M6JN71
Locus tag M5D10_RS12270 Protein ID WP_004462036.1
Coordinates 2693194..2693442 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5D10_RS12250 (M5D10_12225) 2688380..2688769 - 390 WP_002762646.1 response regulator -
M5D10_RS12255 (M5D10_12230) 2688889..2690598 - 1710 WP_004462031.1 EAL domain-containing protein -
M5D10_RS12260 (M5D10_12235) 2690791..2692401 + 1611 WP_004474711.1 adenylate/guanylate cyclase domain-containing protein -
M5D10_RS12265 (M5D10_12240) 2692866..2693207 - 342 WP_269025250.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
M5D10_RS12270 (M5D10_12245) 2693194..2693442 - 249 WP_004462036.1 ribbon-helix-helix domain-containing protein Antitoxin
M5D10_RS12275 (M5D10_12250) 2693945..2694169 - 225 WP_004466072.1 hypothetical protein -
M5D10_RS12280 (M5D10_12255) 2694166..2694348 - 183 WP_026131333.1 hypothetical protein -
M5D10_RS12285 (M5D10_12260) 2694551..2695654 + 1104 WP_004474734.1 histidinol-phosphate transaminase -
M5D10_RS12290 (M5D10_12265) 2695921..2696061 + 141 WP_004462050.1 hypothetical protein -
M5D10_RS12295 (M5D10_12270) 2696062..2697333 - 1272 WP_004474793.1 replication-associated recombination protein A -
M5D10_RS12300 (M5D10_12275) 2697334..2697558 - 225 WP_004470113.1 hypothetical protein -
M5D10_RS12305 (M5D10_12280) 2697738..2698358 - 621 WP_004462057.1 VTT domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - acpXL 2387053..2830044 442991


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12395.20 Da        Isoelectric Point: 6.3473

>T245017 WP_269025250.1 NZ_CP097245:c2693207-2692866 [Leptospira santarosai]
MTRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNRSNINTIVSIAVTSNLNLSEAPGNVLITKKDSNLSKDSVVDVSQIV
TLDKERFLNKAGKLKTNKLNEVGEGLRLVAGLE

Download         Length: 342 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9385.79 Da        Isoelectric Point: 10.3009

>AT245017 WP_004462036.1 NZ_CP097245:c2693442-2693194 [Leptospira santarosai]
MKTAISIPDELFKTAEKTAKKLGIPRSQLFAKALEEFIRSHSKESVTEKLNKIYDNKSKETKTNLTDLSVESLRKSLKND
SW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB M6JN71

References