Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1850710..1851335 Replicon chromosome
Accession NZ_CP097237
Organism Klebsiella pneumoniae strain

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag M4268_RS08975 Protein ID WP_002882817.1
Coordinates 1850952..1851335 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M4268_RS08970 Protein ID WP_004150355.1
Coordinates 1850710..1850952 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M4268_RS08945 (1846701) 1846701..1847300 + 600 WP_004151865.1 glucose-1-phosphatase -
M4268_RS08950 (1847294) 1847294..1848154 + 861 WP_004146232.1 virulence factor BrkB family protein -
M4268_RS08955 (1848151) 1848151..1848588 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M4268_RS08960 (1848633) 1848633..1849574 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M4268_RS08965 (1849588) 1849588..1850505 - 918 WP_004181612.1 alpha/beta hydrolase -
M4268_RS08970 (1850710) 1850710..1850952 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M4268_RS08975 (1850952) 1850952..1851335 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
M4268_RS08980 (1851509) 1851509..1852438 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M4268_RS08985 (1852435) 1852435..1853070 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M4268_RS08990 (1853067) 1853067..1853969 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T245000 WP_002882817.1 NZ_CP097237:1850952-1851335 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT245000 WP_004150355.1 NZ_CP097237:1850710-1850952 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References