Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 375350..376260 Replicon plasmid p390.4
Accession NZ_CP097229
Organism Klebsiella pneumoniae strain 5

Toxin (Protein)


Gene name - Uniprot ID A0A5Q9LR54
Locus tag M4270_RS27760 Protein ID WP_004181896.1
Coordinates 375790..376260 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag M4270_RS27755 Protein ID WP_004181895.1
Coordinates 375350..375793 (+) Length 148 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M4270_RS27740 (M4270_27730) 371983..373470 + 1488 WP_004178082.1 group II intron reverse transcriptase/maturase -
M4270_RS27745 (M4270_27735) 373556..373978 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
M4270_RS27750 (M4270_27740) 373978..375249 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
M4270_RS27755 (M4270_27745) 375350..375793 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
M4270_RS27760 (M4270_27750) 375790..376260 + 471 WP_004181896.1 RES family NAD+ phosphorylase Toxin
M4270_RS27765 (M4270_27755) 376370..376630 - 261 WP_004026352.1 hypothetical protein -
M4270_RS27770 (M4270_27760) 377353..377511 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
M4270_RS27775 (M4270_27765) 378114..379088 + 975 WP_043907013.1 hypothetical protein -
M4270_RS27780 (M4270_27770) 379286..379663 - 378 WP_004181901.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaNDM-1 / aph(3')-VI / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / dfrA14 / blaOXA-1 / aac(6')-Ib-cr / qnrB1 htpB 1..390420 390420


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-148)

Antitoxin

(30-89)


Sequences


Toxin        


Download         Length: 157 a.a.        Molecular weight: 17549.81 Da        Isoelectric Point: 4.6155

>T244980 WP_004181896.1 NZ_CP097229:375790-376260 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T244980 NZ_CP097229:375790-376260 [Klebsiella pneumoniae]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT244980 WP_004181895.1 NZ_CP097229:375350-375793 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT244980 NZ_CP097229:375350-375793 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A5Q9LR54


Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References