Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 102070..102595 Replicon plasmid pECSF1
Accession NC_013655
Organism Escherichia coli SE15

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag ECSF_RS23790 Protein ID WP_001159871.1
Coordinates 102290..102595 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag ECSF_RS23785 Protein ID WP_000813634.1
Coordinates 102070..102288 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECSF_RS23780 99107..99748 - 642 WP_000990667.1 hypothetical protein -
ECSF_RS23785 102070..102288 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ECSF_RS23790 102290..102595 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
ECSF_RS23795 102596..103405 + 810 WP_000016979.1 site-specific integrase -
ECSF_RS23800 103543..103818 - 276 WP_000239529.1 hypothetical protein -
ECSF_RS23805 103812..104456 - 645 WP_000633911.1 AAA family ATPase -
ECSF_RS23810 104685..105656 + 972 WP_001103694.1 hypothetical protein -
ECSF_RS23815 105661..106053 + 393 WP_000340835.1 plasmid partitioning/stability family protein -
ECSF_RS23820 106058..107328 - 1271 Protein_117 translesion error-prone DNA polymerase V subunit UmuC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..122345 122345


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T24496 WP_001159871.1 NC_013655:102290-102595 [Escherichia coli SE15]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T24496 NC_013655:102290-102595 [Escherichia coli SE15]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT24496 WP_000813634.1 NC_013655:102070-102288 [Escherichia coli SE15]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT24496 NC_013655:102070-102288 [Escherichia coli SE15]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References