24495

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4663529..4664105 Replicon chromosome
Accession NC_013654
Organism Escherichia coli SE15

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag ECSF_RS22835 Protein ID WP_001297643.1
Coordinates 4663529..4663816 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag ECSF_RS22840 Protein ID WP_000063156.1
Coordinates 4663803..4664105 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECSF_RS22815 4659782..4661062 - 1281 WP_001037382.1 DUF445 domain-containing protein -
ECSF_RS22820 4661103..4662335 - 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
ECSF_RS25515 4662481..4662576 - 96 Protein_4310 dienelactone hydrolase family protein -
ECSF_RS25520 4662633..4663330 + 698 Protein_4311 IS1 family transposase -
ECSF_RS22835 4663529..4663816 + 288 WP_001297643.1 BrnT family toxin Toxin
ECSF_RS22840 4663803..4664105 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
ECSF_RS22845 4664139..4665095 - 957 WP_001297640.1 GTPase -
ECSF_RS22850 4665106..4665309 - 204 WP_000467859.1 YbdD/YjiX family protein -
ECSF_RS22855 4665359..4667509 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4663121..4663330 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T24495 WP_001297643.1 NC_013654:4663529-4663816 [Escherichia coli SE15]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T24495 NC_013654:4663529-4663816 [Escherichia coli SE15]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT24495 WP_000063156.1 NC_013654:4663803-4664105 [Escherichia coli SE15]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT24495 NC_013654:4663803-4664105 [Escherichia coli SE15]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References