Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 5015299..5016109 Replicon chromosome
Accession NZ_CP097220
Organism Klebsiella pneumoniae strain 12

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag M4272_RS24240 Protein ID WP_004178461.1
Coordinates 5015576..5016109 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag M4272_RS24235 Protein ID WP_002887278.1
Coordinates 5015299..5015565 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M4272_RS24215 (5011171) 5011171..5011515 - 345 WP_004197273.1 cation efflux system protein CusF -
M4272_RS24220 (5011533) 5011533..5012918 - 1386 WP_004178457.1 efflux transporter outer membrane subunit -
M4272_RS24225 (5013090) 5013090..5013773 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
M4272_RS24230 (5013763) 5013763..5015196 + 1434 WP_004178459.1 Cu(+)/Ag(+) sensor histidine kinase -
M4272_RS24235 (5015299) 5015299..5015565 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
M4272_RS24240 (5015576) 5015576..5016109 + 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
M4272_RS24245 (5016157) 5016157..5017278 - 1122 WP_002887282.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T244944 WP_004178461.1 NZ_CP097220:5015576-5016109 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT244944 WP_002887278.1 NZ_CP097220:5015299-5015565 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References