Detailed information of TA system TA024494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4623798..4624618
• Replicon: chromosome
• Accession: NC_013654
• Organism: Escherichia coli SE15

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: ECSF_RS22600
• Protein ID: WP_001054379.1
• Coordinates: 4624361..4624618 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: ECSF_RS22595
• Protein ID: WP_000123957.1
• Coordinates: 4623798..4624349 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSF_RS22570	4619492..4620805	-	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-
ECSF_RS22575	4620817..4621095	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
ECSF_RS22580	4621092..4622213	-	1122	WP_000010833.1	M42 family metallopeptidase	-
ECSF_RS26330	4622458..4622574	-	117	Protein_4267	VOC family protein	-
ECSF_RS22585	4622612..4622872	-	261	WP_000077645.1	hypothetical protein	-
ECSF_RS22590	4623000..4623746	-	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
ECSF_RS22595	4623798..4624349	-	552	WP_000123957.1	N-acetyltransferase	Antitoxin
ECSF_RS22600	4624361..4624618	-	258	WP_001054379.1	YjhX family toxin	Toxin
ECSF_RS22605	4624995..4625294	-	300	WP_001332034.1	GNAT family N-acetyltransferase	-
ECSF_RS22610	4625372..4626103	+	732	Protein_4273	DNA helicase	-
ECSF_RS22615	4626133..4627746	-	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
ECSF_RS22620	4627777..4628127	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
ECSF_RS22625	4628124..4628549	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
ECSF_RS22630	4628635..4629138	+	504	Protein_4277	DNA helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T24494 WP_001054379.1 NC_013654:c4624618-4624361 [Escherichia coli SE15]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T24494 NC_013654:c4624618-4624361 [Escherichia coli SE15]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT24494 WP_000123957.1 NC_013654:c4624349-4623798 [Escherichia coli SE15]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT24494 NC_013654:c4624349-4623798 [Escherichia coli SE15]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030