Detailed information of TA system TA024493

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4592415..4593249
• Replicon: chromosome
• Accession: NC_013654
• Organism: Escherichia coli SE15

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1J6XFW9
• Locus tag: ECSF_RS22450
• Protein ID: WP_000854770.1
• Coordinates: 4592872..4593249 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M1N885
• Locus tag: ECSF_RS22445
• Protein ID: WP_001280950.1
• Coordinates: 4592415..4592783 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSF_RS22400	4587567..4589468	+	1902	Protein_4234	Ag43/Cah family autotransporter adhesin	-
ECSF_RS22405	4589539..4589697	+	159	WP_001323397.1	DUF905 family protein	-
ECSF_RS22415	4589852..4590670	+	819	WP_001234712.1	DUF945 domain-containing protein	-
ECSF_RS22425	4591012..4591476	+	465	WP_000855061.1	antirestriction protein	-
ECSF_RS22430	4591492..4591968	+	477	WP_001186779.1	RadC family protein	-
ECSF_RS22435	4592031..4592252	+	222	WP_001220314.1	DUF987 domain-containing protein	-
ECSF_RS22445	4592415..4592783	+	369	WP_001280950.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECSF_RS22450	4592872..4593249	+	378	WP_000854770.1	TA system toxin CbtA family protein	Toxin
ECSF_RS22455	4593246..4593734	+	489	WP_000761690.1	hypothetical protein	-
ECSF_RS22460	4593751..4593927	+	177	WP_000839286.1	DUF957 domain-containing protein	-
ECSF_RS26070	4594549..4594725	+	177	Protein_4244	helix-turn-helix domain-containing protein	-
ECSF_RS22470	4595498..4597138	+	1641	WP_001332039.1	Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4544427..4604895	60468

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14196.37 Da
• Isoelectric Point: 8.7438

>T24493 WP_000854770.1 NC_013654:4592872-4593249 [Escherichia coli SE15]
MKTLPDTHVRKVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T24493 NC_013654:4592872-4593249 [Escherichia coli SE15]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13737.56 Da
• Isoelectric Point: 7.0268

>AT24493 WP_001280950.1 NC_013654:4592415-4592783 [Escherichia coli SE15]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAFYPTSETKK

Nucleotide

• Length: 369 bp

>AT24493 NC_013654:4592415-4592783 [Escherichia coli SE15]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1J6XFW9

Antitoxin

• AlphaFold DB: A0A1M1N885