Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4124549..4125125 Replicon chromosome
Accession NZ_CP097213
Organism Escherichia coli strain BE311

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag M3O69_RS19935 Protein ID WP_001297643.1
Coordinates 4124838..4125125 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag M3O69_RS19930 Protein ID WP_000063156.1
Coordinates 4124549..4124851 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M3O69_RS19915 (4121145) 4121145..4123295 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
M3O69_RS19920 (4123345) 4123345..4123548 + 204 WP_000467859.1 YbdD/YjiX family protein -
M3O69_RS19925 (4123559) 4123559..4124515 + 957 WP_001297640.1 GTPase -
M3O69_RS19930 (4124549) 4124549..4124851 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
M3O69_RS19935 (4124838) 4124838..4125125 - 288 WP_001297643.1 BrnT family toxin Toxin
M3O69_RS19940 (4125324) 4125324..4126021 - 698 Protein_3897 IS1 family transposase -
M3O69_RS19945 (4126078) 4126078..4126173 + 96 Protein_3898 dienelactone hydrolase family protein -
M3O69_RS19950 (4126319) 4126319..4127551 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
M3O69_RS19955 (4127592) 4127592..4128872 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T244922 WP_001297643.1 NZ_CP097213:c4125125-4124838 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT244922 WP_000063156.1 NZ_CP097213:c4124851-4124549 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References