Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 510589..511226 Replicon chromosome
Accession NZ_CP097208
Organism Bacillus velezensis strain N3

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag M2893_RS02475 Protein ID WP_003156187.1
Coordinates 510876..511226 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag M2893_RS02470 Protein ID WP_003156188.1
Coordinates 510589..510870 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2893_RS02450 (506954) 506954..507553 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
M2893_RS02455 (507646) 507646..508011 + 366 WP_014304402.1 holo-ACP synthase -
M2893_RS02460 (508176) 508176..509183 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
M2893_RS02465 (509300) 509300..510469 + 1170 WP_003156189.1 alanine racemase -
M2893_RS02470 (510589) 510589..510870 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
M2893_RS02475 (510876) 510876..511226 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
M2893_RS02480 (511344) 511344..512165 + 822 WP_014304404.1 STAS domain-containing protein -
M2893_RS02485 (512170) 512170..512535 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
M2893_RS02490 (512538) 512538..512939 + 402 WP_003156178.1 anti-sigma regulatory factor -
M2893_RS02495 (512951) 512951..513958 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
M2893_RS02500 (514022) 514022..514351 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
M2893_RS02505 (514348) 514348..514830 + 483 WP_003156173.1 anti-sigma B factor RsbW -
M2893_RS02510 (514796) 514796..515584 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
M2893_RS02515 (515584) 515584..516186 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T244900 WP_003156187.1 NZ_CP097208:510876-511226 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT244900 WP_003156188.1 NZ_CP097208:510589-510870 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References