Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3552109..3552909 Replicon chromosome
Accession NC_013654
Organism Escherichia coli SE15

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag ECSF_RS17465 Protein ID WP_000342452.1
Coordinates 3552109..3552636 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag ECSF_RS17470 Protein ID WP_001277107.1
Coordinates 3552643..3552909 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECSF_RS17440 3547185..3547952 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
ECSF_RS17445 3547949..3549226 - 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
ECSF_RS17450 3549223..3550149 - 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
ECSF_RS17455 3550197..3551306 - 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
ECSF_RS17460 3551729..3552112 + 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
ECSF_RS17465 3552109..3552636 - 528 WP_000342452.1 GNAT family N-acetyltransferase Toxin
ECSF_RS17470 3552643..3552909 - 267 WP_001277107.1 DUF1778 domain-containing protein Antitoxin
ECSF_RS17475 3553058..3554161 - 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
ECSF_RS17480 3554432..3555286 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
ECSF_RS17485 3555531..3556589 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
ECSF_RS17490 3556582..3557250 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T24490 WP_000342452.1 NC_013654:c3552636-3552109 [Escherichia coli SE15]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T24490 NC_013654:c3552636-3552109 [Escherichia coli SE15]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT24490 WP_001277107.1 NC_013654:c3552909-3552643 [Escherichia coli SE15]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT24490 NC_013654:c3552909-3552643 [Escherichia coli SE15]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References