Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5187480..5188105 Replicon chromosome
Accession NZ_CP097190
Organism Klebsiella pneumoniae strain 15-652

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag M4I39_RS25115 Protein ID WP_019705794.1
Coordinates 5187480..5187863 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M4I39_RS25120 Protein ID WP_004150355.1
Coordinates 5187863..5188105 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M4I39_RS25100 (5184846) 5184846..5185748 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M4I39_RS25105 (5185745) 5185745..5186380 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M4I39_RS25110 (5186377) 5186377..5187306 + 930 WP_274486433.1 formate dehydrogenase accessory protein FdhE -
M4I39_RS25115 (5187480) 5187480..5187863 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
M4I39_RS25120 (5187863) 5187863..5188105 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M4I39_RS25125 (5188310) 5188310..5189227 + 918 WP_004146235.1 alpha/beta hydrolase -
M4I39_RS25130 (5189242) 5189242..5190183 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M4I39_RS25135 (5190228) 5190228..5190665 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M4I39_RS25140 (5190662) 5190662..5191522 - 861 WP_004146232.1 virulence factor BrkB family protein -
M4I39_RS25145 (5191516) 5191516..5192115 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T244895 WP_019705794.1 NZ_CP097190:c5187863-5187480 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT244895 WP_004150355.1 NZ_CP097190:c5188105-5187863 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References