Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4568682..4569492 Replicon chromosome
Accession NZ_CP097190
Organism Klebsiella pneumoniae strain 15-652

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag M4I39_RS22235 Protein ID WP_004178461.1
Coordinates 4568682..4569215 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag M4I39_RS22240 Protein ID WP_002887278.1
Coordinates 4569226..4569492 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M4I39_RS22230 (4567513) 4567513..4568634 + 1122 WP_004186850.1 cupin domain-containing protein -
M4I39_RS22235 (4568682) 4568682..4569215 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
M4I39_RS22240 (4569226) 4569226..4569492 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
M4I39_RS22245 (4569595) 4569595..4571028 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
M4I39_RS22250 (4571018) 4571018..4571701 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
M4I39_RS22255 (4571874) 4571874..4573259 + 1386 WP_064152126.1 efflux transporter outer membrane subunit -
M4I39_RS22260 (4573277) 4573277..4573621 + 345 WP_064152125.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T244892 WP_004178461.1 NZ_CP097190:c4569215-4568682 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT244892 WP_002887278.1 NZ_CP097190:c4569492-4569226 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References