Detailed information of TA system TA244880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 21722..22269
• Replicon: plasmid pYUSHP31-2
• Accession: NZ_CP097183
• Organism: Escherichia coli strain SH21PTE31

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: M4R35_RS23615
• Protein ID: WP_244461891.1
• Coordinates: 21722..21955 (-)
• Length: 78 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A829FT18
• Locus tag: M4R35_RS23620
• Protein ID: WP_000079938.1
• Coordinates: 22000..22269 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M4R35_RS23585	17034..17324	-	291	WP_000648897.1	hypothetical protein	-
M4R35_RS23590	17324..18448	-	1125	WP_032174239.1	DUF4238 domain-containing protein	-
M4R35_RS23595	18483..20006	-	1524	WP_000921251.1	hypothetical protein	-
M4R35_RS23600	20297..20701	-	405	WP_240725492.1	hypothetical protein	-
M4R35_RS23605	20621..21318	+	698	WP_240725490.1	IS1 family transposase	-
M4R35_RS23610	21533..21664	-	132	Protein_23	IS3 family transposase	-
M4R35_RS23615	21722..21955	-	234	WP_244461891.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M4R35_RS23620	22000..22269	-	270	WP_000079938.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
M4R35_RS23625	23004..23984	-	981	WP_071940815.1	IS5-like element ISKpn26 family transposase	-
M4R35_RS23630	24038..24349	+	312	WP_024191312.1	hypothetical protein	-
M4R35_RS23635	24439..25524	-	1086	WP_001148851.1	iron-containing alcohol dehydrogenase family protein	-
M4R35_RS23640	25558..26715	-	1158	WP_000217345.1	alpha-hydroxy acid oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..64527	64527
	-	inside	IScluster/Tn	-	-	10165..38775	28610

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (1-76)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 78 a.a.
• Molecular weight: 8940.20 Da
• Isoelectric Point: 6.7231

>T244880 WP_244461891.1 NZ_CP097183:c21955-21722 [Escherichia coli]
IREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGARELVIHPHFVLVYEVDSQWEKVYILRVLHTTQKWP

Nucleotide

• Length: 234 bp

>T244880 NZ_CP097183:c21955-21722 [Escherichia coli]
ATTCGTGAGTACATTGCGGAGCAGAACCTGATGGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCT
GGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTGTGGAAGGTGCCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGG
TTTATGAGGTCGATAGCCAGTGGGAAAAAGTGTATATCCTGCGTGTATTGCATACAACACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10251.64 Da
• Isoelectric Point: 10.1734

>AT244880 WP_000079938.1 NZ_CP097183:c22269-22000 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLKRGDVLPHDMVESSAAAWRD
EMSRKVADK

Nucleotide

• Length: 270 bp

>AT244880 NZ_CP097183:c22269-22000 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCAAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A829FT18