Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4232552..4233049 Replicon chromosome
Accession NZ_CP097181
Organism Escherichia coli strain SH21PTE31

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag M4R35_RS20720 Protein ID WP_001173343.1
Coordinates 4232552..4232725 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag M4R35_RS20725 Protein ID WP_000398619.1
Coordinates 4232753..4233049 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M4R35_RS20710 (4229217) 4229217..4230674 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
M4R35_RS20715 (4230911) 4230911..4232428 + 1518 WP_001295074.1 lysine--tRNA ligase -
M4R35_RS20720 (4232552) 4232552..4232725 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
M4R35_RS20725 (4232753) 4232753..4233049 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
M4R35_RS20730 (4233276) 4233276..4233548 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
M4R35_RS20735 (4233560) 4233560..4233790 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
M4R35_RS20740 (4233971) 4233971..4235602 + 1632 WP_001565376.1 sensor histidine kinase -
M4R35_RS20745 (4235599) 4235599..4236318 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T244877 WP_001173343.1 NZ_CP097181:c4232725-4232552 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT244877 WP_000398619.1 NZ_CP097181:c4233049-4232753 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References