Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4216583..4217159 Replicon chromosome
Accession NZ_CP097170
Organism Escherichia coli strain AR13438

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag M3M51_RS20330 Protein ID WP_001131965.1
Coordinates 4216872..4217159 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag M3M51_RS20325 Protein ID WP_000063158.1
Coordinates 4216583..4216885 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M3M51_RS20305 (4211691) 4211691..4212911 + 1221 WP_000873756.1 amino acid deaminase -
M3M51_RS20310 (4213179) 4213179..4215329 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
M3M51_RS20315 (4215379) 4215379..4215582 + 204 WP_000467859.1 YbdD/YjiX family protein -
M3M51_RS20320 (4215593) 4215593..4216549 + 957 WP_001361361.1 GTPase -
M3M51_RS20325 (4216583) 4216583..4216885 - 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
M3M51_RS20330 (4216872) 4216872..4217159 - 288 WP_001131965.1 BrnT family toxin Toxin
M3M51_RS20335 (4217356) 4217356..4218522 + 1167 WP_000800823.1 restriction endonuclease -
M3M51_RS20340 (4218567) 4218567..4218740 + 174 Protein_3986 type I restriction-modification system subunit M N-terminal domain-containing protein -
M3M51_RS20345 (4218734) 4218734..4219402 + 669 Protein_3987 DUF3387 domain-containing protein -
M3M51_RS20350 (4219617) 4219617..4219955 + 339 WP_000132633.1 endoribonuclease SymE -
M3M51_RS20355 (4220043) 4220043..4220090 - 48 WP_229017048.1 hypothetical protein -
M3M51_RS20360 (4220338) 4220338..4221750 + 1413 WP_272733520.1 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T244848 WP_001131965.1 NZ_CP097170:c4217159-4216872 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT244848 WP_000063158.1 NZ_CP097170:c4216885-4216583 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References