Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1319164..1320080 Replicon chromosome
Accession NZ_CP097130
Organism Bacillus subtilis strain S16

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag M2M89_RS06875 Protein ID WP_003244695.1
Coordinates 1319334..1320080 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag M2M89_RS06870 Protein ID WP_003232646.1
Coordinates 1319164..1319334 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2M89_RS06835 (1316027) 1316027..1316356 + 330 WP_041850928.1 XkdW family protein -
M2M89_RS06840 (1316353) 1316353..1316517 + 165 WP_041850927.1 XkdX family protein -
M2M89_RS06845 (1316561) 1316561..1317400 + 840 WP_041850926.1 phage-like element PBSX protein XepA -
M2M89_RS06850 (1317453) 1317453..1317722 + 270 WP_003232655.1 hemolysin XhlA family protein -
M2M89_RS06855 (1317735) 1317735..1317998 + 264 WP_003232653.1 phage holin -
M2M89_RS06860 (1318011) 1318011..1318904 + 894 WP_041850925.1 N-acetylmuramoyl-L-alanine amidase -
M2M89_RS06865 (1318941) 1318941..1319078 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
M2M89_RS06870 (1319164) 1319164..1319334 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
M2M89_RS06875 (1319334) 1319334..1320080 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
M2M89_RS06880 (1320190) 1320190..1321191 - 1002 WP_003232642.1 inorganic phosphate transporter -
M2M89_RS06885 (1321204) 1321204..1321821 - 618 WP_003218470.1 DUF47 domain-containing protein -
M2M89_RS06890 (1322097) 1322097..1323413 - 1317 WP_041850924.1 serine/threonine exchanger -
M2M89_RS06895 (1323801) 1323801..1324751 + 951 WP_041344800.1 ring-cleaving dioxygenase -
M2M89_RS06900 (1324852) 1324852..1324998 + 147 WP_120363335.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T244822 WP_003244695.1 NZ_CP097130:c1320080-1319334 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT244822 WP_003232646.1 NZ_CP097130:c1319334-1319164 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References