Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 520987..521623 Replicon chromosome
Accession NZ_CP097130
Organism Bacillus subtilis strain S16

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag M2M89_RS02625 Protein ID WP_003156187.1
Coordinates 521273..521623 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag M2M89_RS02620 Protein ID WP_003225183.1
Coordinates 520987..521268 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2M89_RS02600 (517347) 517347..517946 - 600 WP_041850724.1 rhomboid family intramembrane serine protease -
M2M89_RS02605 (518041) 518041..518406 + 366 WP_015252768.1 holo-ACP synthase -
M2M89_RS02610 (518572) 518572..519588 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
M2M89_RS02615 (519702) 519702..520871 + 1170 WP_015252766.1 alanine racemase -
M2M89_RS02620 (520987) 520987..521268 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
M2M89_RS02625 (521273) 521273..521623 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
M2M89_RS02630 (521738) 521738..522562 + 825 WP_128441371.1 RsbT co-antagonist protein RsbRA -
M2M89_RS02635 (522567) 522567..522932 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
M2M89_RS02640 (522936) 522936..523337 + 402 WP_003225192.1 serine/threonine-protein kinase RsbT -
M2M89_RS02645 (523349) 523349..524356 + 1008 WP_014478900.1 phosphoserine phosphatase RsbU -
M2M89_RS02650 (524425) 524425..524754 + 330 WP_014475877.1 anti-sigma factor antagonist RsbV -
M2M89_RS02655 (524751) 524751..525233 + 483 WP_021481702.1 anti-sigma B factor RsbW -
M2M89_RS02660 (525199) 525199..525987 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
M2M89_RS02665 (525987) 525987..526586 + 600 WP_021481701.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T244821 WP_003156187.1 NZ_CP097130:521273-521623 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT244821 WP_003225183.1 NZ_CP097130:520987-521268 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References