244792

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3670607..3671286 Replicon chromosome
Accession NZ_CP097107
Organism Citrobacter freundii strain GMU8049

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag M3L74_RS18505 Protein ID WP_003031349.1
Coordinates 3670945..3671286 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag M3L74_RS18500 Protein ID WP_003031347.1
Coordinates 3670607..3670924 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M3L74_RS18475 (3666700) 3666700..3668697 - 1998 WP_016245729.1 choline BCCT transporter BetT -
M3L74_RS18480 (3669245) 3669245..3669392 + 148 Protein_3557 hypothetical protein -
M3L74_RS18485 (3669406) 3669406..3669867 + 462 WP_003031344.1 antirestriction protein -
M3L74_RS18490 (3669883) 3669883..3670359 + 477 WP_003031345.1 RadC family protein -
M3L74_RS18495 (3670368) 3670368..3670589 + 222 WP_003031346.1 DUF987 domain-containing protein -
M3L74_RS18500 (3670607) 3670607..3670924 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
M3L74_RS18505 (3670945) 3670945..3671286 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
M3L74_RS18515 (3671916) 3671916..3672224 - 309 WP_003838771.1 isoprenylcysteine carboxylmethyltransferase family protein -
M3L74_RS18520 (3672573) 3672573..3673112 - 540 Protein_3564 EAL domain-containing protein -
M3L74_RS18525 (3673086) 3673086..3673783 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
M3L74_RS18530 (3673777) 3673777..3674499 - 723 WP_249018276.1 winged helix-turn-helix domain-containing protein -
M3L74_RS18535 (3674644) 3674644..3675339 - 696 WP_249018277.1 fimbria/pilus periplasmic chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3656248..3682140 25892
- flank IS/Tn - - 3673406..3673783 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T244792 WP_003031349.1 NZ_CP097107:3670945-3671286 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT244792 WP_003031347.1 NZ_CP097107:3670607-3670924 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References