Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 422110..423733 Replicon chromosome
Accession NZ_CP097107
Organism Citrobacter freundii strain GMU8049

Toxin (Protein)


Gene name hipA Uniprot ID -
Locus tag M3L74_RS02435 Protein ID WP_103798313.1
Coordinates 422408..423733 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5V9NJV0
Locus tag M3L74_RS02430 Protein ID WP_024143021.1
Coordinates 422110..422403 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M3L74_RS02405 (417435) 417435..420443 - 3009 WP_249018339.1 Tn3 family transposase -
M3L74_RS02410 (420602) 420602..420745 + 144 WP_225389218.1 recombinase family protein -
M3L74_RS02415 (420790) 420790..421035 + 246 Protein_409 transposase -
M3L74_RS02420 (421103) 421103..421432 + 330 Protein_410 helix-turn-helix domain-containing protein -
M3L74_RS02425 (421380) 421380..421709 + 330 WP_247753930.1 hypothetical protein -
M3L74_RS02430 (422110) 422110..422403 + 294 WP_024143021.1 helix-turn-helix transcriptional regulator Antitoxin
M3L74_RS02435 (422408) 422408..423733 + 1326 WP_103798313.1 type II toxin-antitoxin system HipA family toxin Toxin
M3L74_RS02440 (423794) 423794..424000 + 207 WP_000134171.1 hypothetical protein -
M3L74_RS02445 (424102) 424102..424512 + 411 WP_000985911.1 hypothetical protein -
M3L74_RS02450 (424525) 424525..425340 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
M3L74_RS02455 (425423) 425423..426202 + 780 Protein_417 IS5-like element ISKpn26 family transposase -
M3L74_RS02460 (426286) 426286..426726 + 441 WP_004189163.1 transposase -
M3L74_RS02465 (426723) 426723..427073 + 351 WP_004189161.1 IS66 family insertion sequence element accessory protein TnpB -
M3L74_RS02470 (427104) 427104..428696 + 1593 WP_004902302.1 IS66-like element ISKox1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 417435..428696 11261


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(155-404)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-70)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.27 Da        Isoelectric Point: 5.8810

>T244783 WP_103798313.1 NZ_CP097107:422408-423733 [Citrobacter freundii]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWYEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLDQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10990.39 Da        Isoelectric Point: 9.9492

>AT244783 WP_024143021.1 NZ_CP097107:422110-422403 [Citrobacter freundii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSVLGVKISFSSATTSSERK
QTEEIYKLNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A5V9NJV0

References