Detailed information of TA system TA244780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4051179..4052221
• Replicon: chromosome
• Accession: NZ_CP097103
• Organism: Pseudomonas sp. NY11382

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: M2J80_RS18470
• Protein ID: WP_003153636.1
• Coordinates: 4051179..4051754 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: M2J80_RS18475
• Protein ID: WP_003050245.1
• Coordinates: 4051751..4052221 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M2J80_RS18445 (M2J80_18430)	4047617..4048342	-	726	WP_003050273.1	CBASS effector endonuclease NucC	-
M2J80_RS18450 (M2J80_18435)	4048381..4049283	-	903	WP_003153640.1	CBASS oligonucleotide cyclase	-
M2J80_RS18455 (M2J80_18440)	4049283..4049783	-	501	WP_003090159.1	type III CBASS phage resistance system CD-NTase-associated protein Cap7	-
M2J80_RS18460 (M2J80_18445)	4049780..4050250	-	471	WP_003153638.1	type III CBASS phage resistance system CD-NTase-associated protein Cap8	-
M2J80_RS18465 (M2J80_18450)	4050247..4051161	-	915	WP_003050256.1	AAA family ATPase	-
M2J80_RS18470 (M2J80_18455)	4051179..4051754	-	576	WP_003153636.1	PIN domain-containing protein	Toxin
M2J80_RS18475 (M2J80_18460)	4051751..4052221	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
M2J80_RS18480 (M2J80_18465)	4052425..4052808	+	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
M2J80_RS18485 (M2J80_18470)	4052805..4053038	+	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
M2J80_RS18490 (M2J80_18475)	4053055..4053414	+	360	WP_003153634.1	TIGR03745 family integrating conjugative element membrane protein	-
M2J80_RS18495 (M2J80_18480)	4053427..4053837	+	411	WP_003821108.1	TIGR03750 family conjugal transfer protein	-
M2J80_RS18500 (M2J80_18485)	4053834..4054526	+	693	WP_012614071.1	TIGR03746 family integrating conjugative element protein	-
M2J80_RS18505 (M2J80_18490)	4054523..4055434	+	912	WP_004350604.1	TIGR03749 family integrating conjugative element protein	-
M2J80_RS18510 (M2J80_18495)	4055424..4056842	+	1419	WP_004350605.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3997873..4115593	117720

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T244780 WP_003153636.1 NZ_CP097103:c4051754-4051179 [Pseudomonas sp. NY11382]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T244780 NZ_CP097103:c4051754-4051179 [Pseudomonas sp. NY11382]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAATCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAACGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT244780 WP_003050245.1 NZ_CP097103:c4052221-4051751 [Pseudomonas sp. NY11382]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT244780 NZ_CP097103:c4052221-4051751 [Pseudomonas sp. NY11382]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68