Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 193569..194305 Replicon plasmid pKP133-1
Accession NZ_CP097077
Organism Klebsiella pneumoniae strain KP133

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag M3I84_RS26280 Protein ID WP_004098919.1
Coordinates 193569..194051 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag M3I84_RS26285 Protein ID WP_004213599.1
Coordinates 194039..194305 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M3I84_RS26260 (M3I84_26230) 188923..190269 + 1347 WP_011154555.1 dihydroorotase -
M3I84_RS26265 (M3I84_26235) 190333..191355 + 1023 WP_131079334.1 porphobilinogen synthase -
M3I84_RS26270 (M3I84_26240) 191530..191925 + 396 Protein_214 IS3 family transposase -
M3I84_RS26275 (M3I84_26245) 191921..193148 - 1228 Protein_215 IS3 family transposase -
M3I84_RS26280 (M3I84_26250) 193569..194051 - 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
M3I84_RS26285 (M3I84_26255) 194039..194305 - 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
M3I84_RS26290 (M3I84_26260) 194500..194730 - 231 WP_004213598.1 hypothetical protein -
M3I84_RS26295 (M3I84_26265) 194744..194947 - 204 WP_004213596.1 HHA domain-containing protein -
M3I84_RS26300 (M3I84_26270) 195008..195502 - 495 WP_004213594.1 hypothetical protein -
M3I84_RS26305 (M3I84_26275) 195544..198513 - 2970 WP_004213592.1 Tn3 family transposase -
M3I84_RS26310 (M3I84_26280) 198516..199073 - 558 WP_004213590.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / ARR-3 / blaKPC-2 / dfrA14 iroB / iroC / iroD / iroN / rmpA / rmpA2 / rmpA / iutA / iucD / iucC / iucB / iucA 1..296587 296587
- inside IScluster/Tn aac(6')-Ib-cr / ARR-3 / blaKPC-2 - 191921..222476 30555


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T244730 WP_004098919.1 NZ_CP097077:c194051-193569 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT244730 WP_004213599.1 NZ_CP097077:c194305-194039 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References