Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5144526..5145151 Replicon chromosome
Accession NZ_CP097076
Organism Klebsiella pneumoniae strain KP133

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag M3I84_RS24835 Protein ID WP_002882817.1
Coordinates 5144526..5144909 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M3I84_RS24840 Protein ID WP_004150355.1
Coordinates 5144909..5145151 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M3I84_RS24820 (5141892) 5141892..5142794 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M3I84_RS24825 (5142791) 5142791..5143426 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M3I84_RS24830 (5143423) 5143423..5144352 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M3I84_RS24835 (5144526) 5144526..5144909 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
M3I84_RS24840 (5144909) 5144909..5145151 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M3I84_RS24845 (5145356) 5145356..5146273 + 918 WP_004178029.1 alpha/beta hydrolase -
M3I84_RS24850 (5146287) 5146287..5147228 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M3I84_RS24855 (5147273) 5147273..5147710 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M3I84_RS24860 (5147707) 5147707..5148567 - 861 WP_004146232.1 virulence factor BrkB family protein -
M3I84_RS24865 (5148561) 5148561..5149160 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T244726 WP_002882817.1 NZ_CP097076:c5144909-5144526 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT244726 WP_004150355.1 NZ_CP097076:c5145151-5144909 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References