Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4556227..4557037 Replicon chromosome
Accession NZ_CP097076
Organism Klebsiella pneumoniae strain KP133

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag M3I84_RS22035 Protein ID WP_004178461.1
Coordinates 4556227..4556760 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag M3I84_RS22040 Protein ID WP_002887278.1
Coordinates 4556771..4557037 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M3I84_RS22030 (4555058) 4555058..4556179 + 1122 WP_020804439.1 cupin domain-containing protein -
M3I84_RS22035 (4556227) 4556227..4556760 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
M3I84_RS22040 (4556771) 4556771..4557037 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
M3I84_RS22045 (4557140) 4557140..4558573 - 1434 WP_020804438.1 Cu(+)/Ag(+) sensor histidine kinase -
M3I84_RS22050 (4558563) 4558563..4559246 - 684 WP_020804437.1 copper response regulator transcription factor CusR -
M3I84_RS22055 (4559418) 4559418..4560803 + 1386 WP_020804440.1 efflux transporter outer membrane subunit -
M3I84_RS22060 (4560821) 4560821..4561165 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T244722 WP_004178461.1 NZ_CP097076:c4556760-4556227 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT244722 WP_002887278.1 NZ_CP097076:c4557037-4556771 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References