244585

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 52286..53423 Replicon plasmid pAT29-a
Accession NZ_CP097047
Organism Enterococcus faecalis strain AT29

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag M2907_RS13695 Protein ID WP_002332783.1
Coordinates 52286..53149 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag M2907_RS13700 Protein ID WP_002326825.1
Coordinates 53151..53423 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2907_RS13670 (M2907_13665) 48612..49349 - 738 WP_181710332.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
M2907_RS13675 (M2907_13670) 49474..49557 - 84 WP_032501549.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
M2907_RS13790 49606..49689 - 84 Protein_60 MLS leader peptide -
M2907_RS13680 (M2907_13675) 50012..50692 + 681 WP_010710189.1 IS6-like element IS1216 family transposase -
M2907_RS13685 (M2907_13680) 50726..51232 - 507 WP_002415429.1 trimethoprim-resistant dihydrofolate reductase DfrG -
M2907_RS13690 (M2907_13685) 51529..51846 - 318 WP_002326830.1 hypothetical protein -
M2907_RS13695 (M2907_13690) 52286..53149 - 864 WP_002332783.1 zeta toxin family protein Toxin
M2907_RS13700 (M2907_13695) 53151..53423 - 273 WP_002326825.1 antitoxin Antitoxin
M2907_RS13705 (M2907_13700) 53441..53656 - 216 WP_001835296.1 peptide-binding protein -
M2907_RS13710 (M2907_13705) 53748..54644 - 897 WP_002326827.1 ParA family protein -
M2907_RS13715 (M2907_13710) 54726..54986 - 261 Protein_68 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
M2907_RS13720 (M2907_13715) 55156..55893 - 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
M2907_RS13725 (M2907_13720) 56018..56101 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
M2907_RS13795 56150..56233 - 84 Protein_71 MLS leader peptide -
M2907_RS13730 (M2907_13725) 56533..56904 - 372 WP_002358205.1 hypothetical protein -
M2907_RS13735 (M2907_13730) 56897..57742 - 846 WP_000239313.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid cat / tet(L) / tet(M) / str / erm(B) / dfrG - 1..58213 58213
- inside IS/Tn erm(B) / dfrG - 48612..55893 7281


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T244585 WP_002332783.1 NZ_CP097047:c53149-52286 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT244585 WP_002326825.1 NZ_CP097047:c53423-53151 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References