244506

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 22933..24070 Replicon plasmid pAT41-a
Accession NZ_CP097032
Organism Enterococcus faecalis strain AT41

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag M2914_RS13315 Protein ID WP_002332783.1
Coordinates 23207..24070 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag M2914_RS13310 Protein ID WP_002326825.1
Coordinates 22933..23205 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2914_RS13270 (M2914_13270) 17949..18032 + 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
M2914_RS13275 (M2914_13275) 18157..18894 + 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
M2914_RS13280 (M2914_13280) 18898..19692 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
M2914_RS13980 20102..20185 + 84 Protein_21 MLS leader peptide -
M2914_RS13285 (M2914_13285) 20234..20317 + 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
M2914_RS13290 (M2914_13290) 20442..21179 + 738 WP_001038789.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
M2914_RS13295 (M2914_13295) 21349..21609 + 261 Protein_24 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
M2914_RS13300 (M2914_13300) 21712..22608 + 897 WP_002326827.1 ParA family protein -
M2914_RS13305 (M2914_13305) 22700..22915 + 216 WP_001835296.1 peptide-binding protein -
M2914_RS13310 (M2914_13310) 22933..23205 + 273 WP_002326825.1 antitoxin Antitoxin
M2914_RS13315 (M2914_13315) 23207..24070 + 864 WP_002332783.1 zeta toxin family protein Toxin
M2914_RS13320 (M2914_13320) 24510..24827 + 318 WP_002326830.1 hypothetical protein -
M2914_RS13325 (M2914_13325) 25039..25323 + 285 WP_000922261.1 hypothetical protein -
M2914_RS13330 (M2914_13330) 25330..27282 + 1953 WP_000163792.1 hypothetical protein -
M2914_RS13335 (M2914_13335) 27354..27851 - 498 WP_000868795.1 trimethoprim-resistant dihydrofolate reductase DfrG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid cat / tet(L) / tet(M) / erm(B) / aph(3')-III / dfrG / aac(6')-aph(2'') bsh 1..75254 75254
- flank IS/Tn erm(B) / aph(3')-III - 16688..21179 4491


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T244506 WP_002332783.1 NZ_CP097032:23207-24070 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT244506 WP_002326825.1 NZ_CP097032:22933-23205 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References