Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 468164..468735 Replicon chromosome
Accession NZ_CP097022
Organism Enterococcus faecium strain AT45b

Toxin (Protein)


Gene name mazF Uniprot ID A0A828ZXV4
Locus tag M2917_RS02215 Protein ID WP_002302307.1
Coordinates 468394..468735 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag M2917_RS02210 Protein ID WP_002323011.1
Coordinates 468164..468394 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2917_RS02185 (M2917_02185) 463653..464981 + 1329 WP_044383738.1 FAD-dependent oxidoreductase -
M2917_RS02190 (M2917_02190) 465003..465629 + 627 WP_002286816.1 cysteine hydrolase -
M2917_RS02195 (M2917_02195) 465812..466393 + 582 WP_002331663.1 TetR/AcrR family transcriptional regulator -
M2917_RS02200 (M2917_02200) 466758..467333 + 576 WP_002331662.1 SOS response-associated peptidase family protein -
M2917_RS02205 (M2917_02205) 467538..467876 - 339 WP_002286804.1 hypothetical protein -
M2917_RS02210 (M2917_02210) 468164..468394 + 231 WP_002323011.1 hypothetical protein Antitoxin
M2917_RS02215 (M2917_02215) 468394..468735 + 342 WP_002302307.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
M2917_RS02220 (M2917_02220) 469585..469770 + 186 WP_002304207.1 hypothetical protein -
M2917_RS02225 (M2917_02225) 470275..470469 + 195 WP_002295146.1 hypothetical protein -
M2917_RS02230 (M2917_02230) 470660..470909 + 250 Protein_442 transposase -
M2917_RS02235 (M2917_02235) 471153..471983 - 831 WP_002286789.1 manganese catalase family protein -
M2917_RS02240 (M2917_02240) 472191..473525 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13288.65 Da        Isoelectric Point: 9.9044

>T244450 WP_002302307.1 NZ_CP097022:468394-468735 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT244450 WP_002323011.1 NZ_CP097022:468164-468394 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXV4

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References