244419

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 59736..60844 Replicon plasmid pAT47b-a
Accession NZ_CP097015
Organism Enterococcus faecium strain AT47b

Toxin (Protein)


Gene name MNTss Uniprot ID A0A3S8RBH6
Locus tag M2920_RS13000 Protein ID WP_071661597.1
Coordinates 59736..60605 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID -
Locus tag M2920_RS13005 Protein ID WP_000205227.1
Coordinates 60620..60844 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2920_RS12965 (M2920_12965) 55092..55325 - 234 WP_002295764.1 addiction module antitoxin -
M2920_RS12970 (M2920_12970) 55647..56732 + 1086 WP_002328898.1 tyrosine recombinase XerS -
M2920_RS12975 (M2920_12975) 56795..57349 - 555 WP_002295761.1 tyrosine-type recombinase/integrase -
M2920_RS12980 (M2920_12980) 57709..58389 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
M2920_RS12985 (M2920_12985) 58467..58859 - 393 WP_000393259.1 GNAT family N-acetyltransferase -
M2920_RS12990 (M2920_12990) 58878..58988 - 111 Protein_71 aminoglycoside 6-adenylyltransferase -
M2920_RS12995 (M2920_12995) 59021..59755 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
M2920_RS13000 (M2920_13000) 59736..60605 - 870 WP_071661597.1 nucleotidyltransferase domain-containing protein Toxin
M2920_RS13005 (M2920_13005) 60620..60844 - 225 WP_000205227.1 helix-turn-helix transcriptional regulator Antitoxin
M2920_RS13010 (M2920_13010) 61262..62563 + 1302 Protein_75 ISL3 family transposase -
M2920_RS13015 (M2920_13015) 62872..63675 - 804 WP_002294514.1 lincosamide nucleotidyltransferase Lnu(B) -
M2920_RS13020 (M2920_13020) 63729..65213 - 1485 WP_002294513.1 ABC-F type ribosomal protection protein Lsa(E) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32902.55 Da        Isoelectric Point: 4.9770

>T244419 WP_071661597.1 NZ_CP097015:c60605-59736 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTE
KVNHIFEVLGISLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8365.68 Da        Isoelectric Point: 4.5510

>AT244419 WP_000205227.1 NZ_CP097015:c60844-60620 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S8RBH6

Antitoxin

Source ID Structure

References