Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 500607..501178 Replicon chromosome
Accession NZ_CP097014
Organism Enterococcus faecium strain AT47b

Toxin (Protein)


Gene name mazF Uniprot ID A0A828ZXV4
Locus tag M2920_RS02360 Protein ID WP_002302307.1
Coordinates 500837..501178 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag M2920_RS02355 Protein ID WP_002323011.1
Coordinates 500607..500837 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2920_RS02330 (M2920_02330) 496096..497424 + 1329 WP_010718318.1 FAD-dependent oxidoreductase -
M2920_RS02335 (M2920_02335) 497446..498072 + 627 WP_002286816.1 cysteine hydrolase -
M2920_RS02340 (M2920_02340) 498255..498836 + 582 WP_002331663.1 TetR/AcrR family transcriptional regulator -
M2920_RS02345 (M2920_02345) 499201..499776 + 576 WP_010718317.1 SOS response-associated peptidase family protein -
M2920_RS02350 (M2920_02350) 499981..500319 - 339 WP_002286804.1 hypothetical protein -
M2920_RS02355 (M2920_02355) 500607..500837 + 231 WP_002323011.1 hypothetical protein Antitoxin
M2920_RS02360 (M2920_02360) 500837..501178 + 342 WP_002302307.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
M2920_RS02365 (M2920_02365) 502028..502213 + 186 WP_002304207.1 hypothetical protein -
M2920_RS02375 (M2920_02375) 503732..503974 + 243 Protein_473 LPXTG cell wall anchor domain-containing protein -
M2920_RS02380 (M2920_02380) 504048..504941 + 894 WP_002326335.1 class C sortase -
M2920_RS02385 (M2920_02385) 505125..505799 + 675 WP_002291233.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element ClpL - 431873..608967 177094


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13288.65 Da        Isoelectric Point: 9.9044

>T244418 WP_002302307.1 NZ_CP097014:500837-501178 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT244418 WP_002323011.1 NZ_CP097014:500607-500837 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXV4

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References