Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 80792..81900 Replicon plasmid pAT48-a
Accession NZ_CP097011
Organism Enterococcus faecalis strain AT48

Toxin (Protein)


Gene name MNTss Uniprot ID -
Locus tag M2921_RS13515 Protein ID WP_078126936.1
Coordinates 80792..81661 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag M2921_RS13520 Protein ID WP_002303393.1
Coordinates 81676..81900 (-) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2921_RS13485 (M2921_13490) 76223..76960 - 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
M2921_RS13490 (M2921_13495) 77085..77168 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
M2921_RS14230 77217..77300 - 84 Protein_86 MLS leader peptide -
M2921_RS13495 (M2921_13500) 77710..78504 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
M2921_RS13500 (M2921_13505) 78597..79139 - 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
M2921_RS13505 (M2921_13510) 79136..80044 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
M2921_RS13510 (M2921_13515) 80077..80811 - 735 WP_000662263.1 class I SAM-dependent methyltransferase -
M2921_RS13515 (M2921_13520) 80792..81661 - 870 WP_078126936.1 nucleotidyltransferase domain-containing protein Toxin
M2921_RS13520 (M2921_13525) 81676..81900 - 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
M2921_RS13525 (M2921_13530) 82043..82864 - 822 Protein_93 recombinase zinc beta ribbon domain-containing protein -
M2921_RS13530 (M2921_13535) 83552..84355 - 804 WP_002294514.1 lincosamide nucleotidyltransferase Lnu(B) -
M2921_RS13535 (M2921_13540) 84409..85894 - 1486 Protein_95 ABC-F type ribosomal protection protein Lsa(E) -
M2921_RS13540 (M2921_13545) 86337..86900 - 564 Protein_96 recombinase zinc beta ribbon domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid cat / tet(L) / tet(M) / erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) / aac(6')-aph(2'') / dfrG - 1..102954 102954
- inside IS/Tn erm(B) / aph(3')-III / ant(6)-Ia / lnu(B) / lsa(E) / aac(6')-aph(2'') / dfrG - 76223..100634 24411


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32835.43 Da        Isoelectric Point: 4.7269

>T244415 WP_078126936.1 NZ_CP097011:c81661-80792 [Enterococcus faecalis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT244415 WP_002303393.1 NZ_CP097011:c81900-81676 [Enterococcus faecalis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References