Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 57367..58504 Replicon plasmid pLS06-1-a
Accession NZ_CP097003
Organism Enterococcus faecalis strain LS06-1

Toxin (Protein)


Gene name zeta Uniprot ID D1MAU9
Locus tag M2926_RS12820 Protein ID WP_002333464.1
Coordinates 57641..58504 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag M2926_RS12815 Protein ID WP_000301765.1
Coordinates 57367..57639 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2926_RS12780 (M2926_12780) 52698..53240 + 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
M2926_RS12785 (M2926_12785) 53333..54127 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
M2926_RS12990 54537..54620 + 84 Protein_58 MLS leader peptide -
M2926_RS12790 (M2926_12790) 54669..54752 + 84 WP_117844860.1 23S rRNA methyltransferase attenuation leader peptide -
M2926_RS12795 (M2926_12795) 54877..55614 + 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
M2926_RS12800 (M2926_12800) 55784..56044 + 261 Protein_61 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
M2926_RS12805 (M2926_12805) 56147..57043 + 897 WP_001809760.1 ParA family protein -
M2926_RS12810 (M2926_12810) 57135..57350 + 216 WP_002321610.1 peptide-binding protein -
M2926_RS12815 (M2926_12815) 57367..57639 + 273 WP_000301765.1 antitoxin Antitoxin
M2926_RS12820 (M2926_12820) 57641..58504 + 864 WP_002333464.1 zeta toxin family protein Toxin
M2926_RS12825 (M2926_12825) 59098..59778 - 681 WP_010710189.1 IS6-like element IS1216 family transposase -
M2926_RS12830 (M2926_12830) 59942..61225 - 1284 WP_002362438.1 hypothetical protein -
M2926_RS12840 (M2926_12840) 62626..63228 - 603 WP_002362434.1 Fic family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid cat / tet(L) / tet(M) / ant(6)-Ia / aph(3')-III / erm(B) - 1..72603 72603
- inside IScluster/Tn ant(6)-Ia / aph(3')-III / erm(B) - 50676..59784 9108


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32370.87 Da        Isoelectric Point: 6.6610

>T244350 WP_002333464.1 NZ_CP097003:57641-58504 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGL

Download         Length: 864 bp

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT244350 WP_000301765.1 NZ_CP097003:57367-57639 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D1MAU9

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References