244329

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3952222..3953059 Replicon chromosome
Accession NZ_CP096976
Organism Escherichia coli strain 17-07187

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag M2894_RS19430 Protein ID WP_000227784.1
Coordinates 3952517..3953059 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag M2894_RS19425 Protein ID WP_001297137.1
Coordinates 3952222..3952533 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2894_RS19400 (3947242) 3947242..3948189 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
M2894_RS19405 (3948211) 3948211..3950202 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
M2894_RS19410 (3950192) 3950192..3950806 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
M2894_RS19415 (3950806) 3950806..3951135 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
M2894_RS19420 (3951147) 3951147..3952037 + 891 WP_000971336.1 heme o synthase -
M2894_RS19425 (3952222) 3952222..3952533 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
M2894_RS19430 (3952517) 3952517..3953059 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
M2894_RS19435 (3953115) 3953115..3954050 - 936 WP_024174603.1 tetratricopeptide repeat protein -
M2894_RS19440 (3954458) 3954458..3955822 + 1365 WP_001000974.1 MFS transporter -
M2894_RS19445 (3955950) 3955950..3956441 - 492 WP_001138904.1 nucleotide binding protein YajQ -
M2894_RS19450 (3956609) 3956609..3957520 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T244329 WP_000227784.1 NZ_CP096976:3952517-3953059 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT244329 WP_001297137.1 NZ_CP096976:3952222-3952533 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References