Detailed information of TA system TA244280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5743830..5744416
• Replicon: chromosome
• Accession: NZ_CP096960
• Organism: Pseudomonas aeruginosa strain NY11254

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: M2I99_RS27110
• Protein ID: WP_003120987.1
• Coordinates: 5744117..5744416 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: M2I99_RS27105
• Protein ID: WP_003448662.1
• Coordinates: 5743830..5744120 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M2I99_RS27090 (M2I99_27030)	5739414..5741303	+	1890	WP_023101908.1	hypothetical protein	-
M2I99_RS27095 (M2I99_27035)	5741300..5743276	+	1977	WP_031691363.1	DEAD/DEAH box helicase	-
M2I99_RS27100 (M2I99_27040)	5743415..5743759	+	345	WP_023082045.1	hypothetical protein	-
M2I99_RS27105 (M2I99_27045)	5743830..5744120	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
M2I99_RS27110 (M2I99_27050)	5744117..5744416	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M2I99_RS27115 (M2I99_27055)	5744618..5745742	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
M2I99_RS27120 (M2I99_27060)	5745742..5747451	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
M2I99_RS27125 (M2I99_27065)	5747455..5748780	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
M2I99_RS27130 (M2I99_27070)	5748770..5749303	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5713061..5796707	83646

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T244280 WP_003120987.1 NZ_CP096960:c5744416-5744117 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T244280 NZ_CP096960:c5744416-5744117 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT244280 WP_003448662.1 NZ_CP096960:c5744120-5743830 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT244280 NZ_CP096960:c5744120-5743830 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCGACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGCGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73