Detailed information of TA system TA024418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2145429..2145958
• Replicon: chromosome
• Accession: NC_013450
• Organism: Staphylococcus aureus subsp. aureus ED98

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAAV_RS10940
• Protein ID: WP_000621175.1
• Coordinates: 2145429..2145791 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAAV_RS10945
• Protein ID: WP_000948331.1
• Coordinates: 2145788..2145958 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAAV_RS10910	2140965..2142284	+	1320	WP_001557163.1	ISL3-like element IS1181 family transposase	-
SAAV_RS10915	2142407..2143177	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
SAAV_RS10920	2143152..2143631	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
SAAV_RS10925	2143633..2143959	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAAV_RS10930	2144078..2145079	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAAV_RS10940	2145429..2145791	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAAV_RS10945	2145788..2145958	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAAV_RS10950	2146043..2147191	-	1149	WP_001281154.1	alanine racemase	-
SAAV_RS10955	2147257..2147616	-	360	WP_000581197.1	holo-ACP synthase	-
SAAV_RS10960	2147620..2148111	-	492	WP_001205912.1	PH domain-containing protein	-
SAAV_RS10965	2148098..2149681	-	1584	WP_001294626.1	PH domain-containing protein	-
SAAV_RS10970	2149674..2150153	-	480	WP_001287087.1	hypothetical protein	-
SAAV_RS10975	2150361..2150921	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2140965..2142284	1319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T24418 WP_000621175.1 NC_013450:c2145791-2145429 [Staphylococcus aureus subsp. aureus ED98]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T24418 NC_013450:c2145791-2145429 [Staphylococcus aureus subsp. aureus ED98]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT24418 WP_000948331.1 NC_013450:c2145958-2145788 [Staphylococcus aureus subsp. aureus ED98]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT24418 NC_013450:c2145958-2145788 [Staphylococcus aureus subsp. aureus ED98]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1