Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-higA/HigB-HigA
Location 343708..344699 Replicon chromosome
Accession NZ_CP096920
Organism Pseudomonas putida strain NY4811

Toxin (Protein)


Gene name relE Uniprot ID -
Locus tag M2J85_RS01515 Protein ID WP_232435437.1
Coordinates 343708..344184 (+) Length 159 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q88R60
Locus tag M2J85_RS01520 Protein ID WP_004575922.1
Coordinates 344226..344699 (+) Length 158 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2J85_RS01495 (M2J85_01490) 338749..340116 - 1368 WP_060539158.1 ATP-binding protein -
M2J85_RS01500 (M2J85_01495) 340118..340837 - 720 WP_004575925.1 response regulator -
M2J85_RS01505 (M2J85_01500) 340978..343275 + 2298 WP_003255779.1 TonB-dependent siderophore receptor -
M2J85_RS01510 (M2J85_01505) 343443..343649 - 207 WP_003255778.1 DUF3079 domain-containing protein -
M2J85_RS01515 (M2J85_01510) 343708..344184 + 477 WP_232435437.1 type II toxin-antitoxin system HigB family toxin Toxin
M2J85_RS01520 (M2J85_01515) 344226..344699 + 474 WP_004575922.1 transcriptional regulator Antitoxin
M2J85_RS01525 (M2J85_01520) 344704..344917 - 214 Protein_294 integrase -
M2J85_RS01530 (M2J85_01525) 345120..345362 + 243 WP_232435436.1 type II toxin-antitoxin system RelE/ParE family toxin -
M2J85_RS01535 (M2J85_01530) 345321..345479 - 159 Protein_296 transcriptional regulator -
M2J85_RS01540 (M2J85_01535) 345474..345689 - 216 Protein_297 integrase -
M2J85_RS01545 (M2J85_01540) 345677..346036 + 360 Protein_298 phage portal protein -
M2J85_RS01550 (M2J85_01545) 346050..346403 - 354 WP_146051591.1 hypothetical protein -
M2J85_RS01555 (M2J85_01550) 346652..347194 - 543 WP_004575918.1 ImmA/IrrE family metallo-endopeptidase -
M2J85_RS01560 (M2J85_01555) 347772..348695 - 924 WP_259537442.1 IS3 family transposase -
M2J85_RS01565 (M2J85_01560) 348692..348985 - 294 WP_060537279.1 transposase -
M2J85_RS01570 (M2J85_01565) 349248..349682 - 435 WP_004577124.1 DUF4145 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(114-152)

Antitoxin

Antitoxin HigA

(43-154)


Sequences

Toxin        


Download         Length: 159 a.a.        Molecular weight: 18459.43 Da        Isoelectric Point: 10.2237

>T244171 WP_232435437.1 NZ_CP096920:343708-344184 [Pseudomonas putida]
MTGINPLAIARSPYIAHPDIWLTDIWIRDIFHPMLVRRDNTMRVITKAAVTKAIEAHGQWKAPLSLWLTTFDRATLRFES
FEQLRQTWVTVSGWSVDRIPHSELRPASRKGPLDIYVFDIKKNECRLIAWLNARTGTLYIKAILSHAEYDKWCRSDIR

Download         Length: 477 bp

Antitoxin


Download         Length: 158 a.a.        Molecular weight: 17229.67 Da        Isoelectric Point: 4.4982

>AT244171 WP_004575922.1 NZ_CP096920:344226-344699 [Pseudomonas putida]
MKRDKIEANGLDAFIANISPMLDGLNTQMATMAQLLAACHDPIKDDAELEARMALIDELYSHADSEGHAAARFAEMVADR
VYEYESESVLVPFASQAEALAFLLSDRGVKQKDLSAIATQSAVSEILNNKRKMTVAQIKGFAEFFSVPVEFFMHGVV

Download         Length: 474 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A2I0CBJ4

References