Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 28114..28807 Replicon plasmid pNY8688-NR
Accession NZ_CP096911
Organism Pseudomonas aeruginosa strain NY8688

Toxin (Protein)


Gene name tad Uniprot ID N2IHR9
Locus tag M2J77_RS32670 Protein ID WP_003151133.1
Coordinates 28430..28807 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID N2IIN5
Locus tag M2J77_RS32665 Protein ID WP_001172026.1
Coordinates 28114..28449 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2J77_RS32615 (M2J77_32540) 23426..23680 - 255 WP_196994195.1 hypothetical protein -
M2J77_RS32620 (M2J77_32545) 23707..23913 - 207 WP_152996423.1 hypothetical protein -
M2J77_RS32625 (M2J77_32550) 24026..24325 - 300 WP_196994194.1 hypothetical protein -
M2J77_RS32630 (M2J77_32555) 24354..24545 - 192 WP_196994193.1 hypothetical protein -
M2J77_RS32635 (M2J77_32560) 24621..24887 - 267 WP_196994192.1 hypothetical protein -
M2J77_RS32640 (M2J77_32565) 24926..25996 - 1071 WP_196994191.1 DNA-binding protein -
M2J77_RS32645 (M2J77_32570) 26268..27017 - 750 Protein_45 DUF3560 domain-containing protein -
M2J77_RS32650 (M2J77_32575) 27057..27398 - 342 WP_025999701.1 hypothetical protein -
M2J77_RS32655 (M2J77_32580) 27414..27740 - 327 WP_000091614.1 hypothetical protein -
M2J77_RS32660 (M2J77_32585) 27764..28099 - 336 WP_000741275.1 hypothetical protein -
M2J77_RS32665 (M2J77_32590) 28114..28449 - 336 WP_001172026.1 helix-turn-helix transcriptional regulator Antitoxin
M2J77_RS32670 (M2J77_32595) 28430..28807 - 378 WP_003151133.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M2J77_RS32675 (M2J77_32600) 28999..29601 + 603 WP_010465829.1 recombinase family protein -
M2J77_RS32680 (M2J77_32605) 29585..32614 + 3030 WP_270145813.1 Tn3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..58793 58793


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T244151 WP_003151133.1 NZ_CP096911:c28807-28430 [Pseudomonas aeruginosa]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT244151 WP_001172026.1 NZ_CP096911:c28449-28114 [Pseudomonas aeruginosa]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A024ELN5

Antitoxin

Source ID Structure
AlphaFold DB A0A024EKI7

References