Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/FR47-DUF1778
Location 2228..3025 Replicon plasmid pCA_RSHH22_1
Accession NZ_CP096906
Organism Citrobacter amalonaticus strain RSHH22

Toxin (Protein)


Gene name KacT Uniprot ID F5S3R9
Locus tag M2R49_RS23420 Protein ID WP_006812498.1
Coordinates 2501..3025 (+) Length 175 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5S3R8
Locus tag M2R49_RS23415 Protein ID WP_006812497.1
Coordinates 2228..2497 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2R49_RS23405 (M2R49_23385) 837..1190 - 354 WP_160859104.1 hypothetical protein -
M2R49_RS23410 (M2R49_23390) 1196..1861 - 666 WP_023315962.1 AAA family ATPase -
M2R49_RS23415 (M2R49_23395) 2228..2497 + 270 WP_006812497.1 DUF1778 domain-containing protein Antitoxin
M2R49_RS23420 (M2R49_23400) 2501..3025 + 525 WP_006812498.1 GNAT family N-acetyltransferase Toxin
M2R49_RS23425 (M2R49_23405) 3052..3216 - 165 WP_006812499.1 hypothetical protein -
M2R49_RS23430 (M2R49_23410) 3209..3460 - 252 WP_000147960.1 hypothetical protein -
M2R49_RS23435 (M2R49_23415) 3462..4154 - 693 WP_006812500.1 membrane protein -
M2R49_RS23440 (M2R49_23420) 4168..4491 - 324 WP_000064173.1 hypothetical protein -
M2R49_RS23445 (M2R49_23425) 4566..5354 - 789 WP_032655902.1 receptor-recognizing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..110958 110958


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 175 a.a.        Molecular weight: 19356.13 Da        Isoelectric Point: 8.9844

>T244142 WP_006812498.1 NZ_CP096906:2501-3025 [Citrobacter amalonaticus]
VANLTIEMFSEEAVYDFSCFDCGETSLNDFLNNRLAQQHSGRILRGYLLLTRDPIPKVMGFYTLSGSCFARNTLPSNTQQ
RKVPYSDAPSVTLGRLAIDKSIQRQGYGETLVAHAMKVVYRASLAVGIYALFVDAKNENAMRFYKQLGFTPLTGDNANSL
FYPTKGIEKLFGGK

Download         Length: 525 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10114.51 Da        Isoelectric Point: 4.8483

>AT244142 WP_006812497.1 NZ_CP096906:2228-2497 [Citrobacter amalonaticus]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNIVMDAISNPPAPNDRLKR
AAERLQSME

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0QWY8

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0QUA3

References