Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4472061..4472558 Replicon chromosome
Accession NZ_CP096902
Organism Escherichia coli strain RSHH22

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag M1Y13_RS21985 Protein ID WP_001173343.1
Coordinates 4472061..4472234 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag M1Y13_RS21990 Protein ID WP_000398619.1
Coordinates 4472262..4472558 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1Y13_RS21975 (4468731) 4468731..4470188 + 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -
M1Y13_RS21980 (4470425) 4470425..4471942 + 1518 WP_001295074.1 lysine--tRNA ligase -
M1Y13_RS21985 (4472061) 4472061..4472234 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
M1Y13_RS21990 (4472262) 4472262..4472558 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
M1Y13_RS21995 (4472785) 4472785..4473057 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
M1Y13_RS22000 (4473069) 4473069..4473299 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
M1Y13_RS22005 (4473480) 4473480..4475111 + 1632 WP_001216461.1 sensor histidine kinase -
M1Y13_RS22010 (4475108) 4475108..4475827 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T244121 WP_001173343.1 NZ_CP096902:c4472234-4472061 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT244121 WP_000398619.1 NZ_CP096902:c4472558-4472262 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References