Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 546948..547584 Replicon chromosome
Accession NZ_CP096889
Organism Bacillus cabrialesii strain TE3

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag EFK13_RS02800 Protein ID WP_003156187.1
Coordinates 547234..547584 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag EFK13_RS02795 Protein ID WP_003225183.1
Coordinates 546948..547229 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EFK13_RS02775 (EFK13_02775) 543308..543907 - 600 WP_129506639.1 rhomboid family intramembrane serine protease -
EFK13_RS02780 (EFK13_02780) 544002..544367 + 366 WP_129506638.1 holo-ACP synthase -
EFK13_RS02785 (EFK13_02785) 544532..545548 + 1017 WP_129506637.1 outer membrane lipoprotein carrier protein LolA -
EFK13_RS02790 (EFK13_02790) 545663..546832 + 1170 WP_129506738.1 alanine racemase -
EFK13_RS02795 (EFK13_02795) 546948..547229 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
EFK13_RS02800 (EFK13_02800) 547234..547584 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EFK13_RS02805 (EFK13_02805) 547700..548524 + 825 WP_129506636.1 RsbT co-antagonist protein RsbRA -
EFK13_RS02810 (EFK13_02810) 548529..548894 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
EFK13_RS02815 (EFK13_02815) 548898..549299 + 402 WP_014112733.1 serine/threonine-protein kinase RsbT -
EFK13_RS02820 (EFK13_02820) 549311..550318 + 1008 WP_129506635.1 phosphoserine phosphatase RsbU -
EFK13_RS02825 (EFK13_02825) 550380..550709 + 330 WP_129506634.1 anti-sigma factor antagonist RsbV -
EFK13_RS02830 (EFK13_02830) 550706..551188 + 483 WP_003234299.1 anti-sigma B factor RsbW -
EFK13_RS02835 (EFK13_02835) 551154..551942 + 789 WP_003225200.1 RNA polymerase sigma factor SigB -
EFK13_RS02840 (EFK13_02840) 551942..552541 + 600 WP_129506633.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T244083 WP_003156187.1 NZ_CP096889:547234-547584 [Bacillus cabrialesii]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT244083 WP_003225183.1 NZ_CP096889:546948-547229 [Bacillus cabrialesii]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References