244080

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 1303..1904 Replicon plasmid unnamed
Accession NZ_CP096854
Organism Lactiplantibacillus plantarum strain LPgoji

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag M1853_RS15525 Protein ID WP_001748110.1
Coordinates 1560..1904 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID Q684P2
Locus tag M1853_RS15520 Protein ID WP_003643337.1
Coordinates 1303..1566 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1853_RS15515 (M1853_15445) 630..1217 - 588 WP_010625610.1 site-specific integrase -
M1853_RS15520 (M1853_15450) 1303..1566 + 264 WP_003643337.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
M1853_RS15525 (M1853_15455) 1560..1904 + 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
M1853_RS15530 (M1853_15460) 2226..3155 + 930 Protein_3 IS30-like element ISLpl1 family transposase -
M1853_RS15535 (M1853_15465) 3255..3623 + 369 WP_003643339.1 hypothetical protein -
M1853_RS15540 (M1853_15470) 3746..3946 + 201 WP_003643340.1 cold-shock protein -
M1853_RS15545 (M1853_15475) 4033..4275 + 243 WP_003643341.1 hypothetical protein -
M1853_RS15550 (M1853_15480) 4919..5443 + 525 WP_003646143.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..7192 7192
- flank IS/Tn - - 2226..2999 773


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T244080 WP_001748110.1 NZ_CP096854:1560-1904 [Lactiplantibacillus plantarum]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T244080 NZ_CP096854:1560-1904 [Lactiplantibacillus plantarum]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCGCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.51 Da        Isoelectric Point: 6.4708

>AT244080 WP_003643337.1 NZ_CP096854:1303-1566 [Lactiplantibacillus plantarum]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWKDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT244080 NZ_CP096854:1303-1566 [Lactiplantibacillus plantarum]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGAAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A6L4ZZT2

References