Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3018772..3019451 Replicon chromosome
Accession NZ_CP096848
Organism Mycobacterium tuberculosis variant bovis strain Mb0486

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag M1778_RS14285 Protein ID WP_003414059.1
Coordinates 3018772..3019188 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag M1778_RS14290 Protein ID WP_003414061.1
Coordinates 3019185..3019451 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1778_RS14265 (M1778_14260) 3014824..3015726 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
M1778_RS14270 (M1778_14265) 3015795..3016547 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
M1778_RS14275 (M1778_14275) 3016791..3017066 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
M1778_RS14280 (M1778_14280) 3017063..3018685 - 1623 WP_003414057.1 class I SAM-dependent DNA methyltransferase -
M1778_RS14285 (M1778_14285) 3018772..3019188 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
M1778_RS14290 (M1778_14290) 3019185..3019451 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
M1778_RS14295 (M1778_14295) 3019477..3019872 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
M1778_RS14300 (M1778_14300) 3019869..3020138 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
M1778_RS14305 (M1778_14305) 3020148..3021242 - 1095 WP_003414068.1 restriction endonuclease subunit S -
M1778_RS14310 (M1778_14310) 3021239..3021658 - 420 Protein_2823 winged helix-turn-helix domain-containing protein -
M1778_RS14315 (M1778_14315) 3021657..3021731 + 75 Protein_2824 hypothetical protein -
M1778_RS14320 (M1778_14320) 3021732..3022211 - 480 WP_003414073.1 dihydrofolate reductase -
M1778_RS14325 (M1778_14325) 3022282..3023082 - 801 WP_003911953.1 thymidylate synthase -
M1778_RS14330 (M1778_14330) 3023238..3023975 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-123)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(16-56)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T244068 WP_003414059.1 NZ_CP096848:c3019188-3018772 [Mycobacterium tuberculosis variant bovis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT244068 WP_003414061.1 NZ_CP096848:c3019451-3019185 [Mycobacterium tuberculosis variant bovis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5SV2

Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References