Detailed information of TA system TA024402

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 3411054..3411649
• Replicon: chromosome
• Accession: NC_013446
• Organism: Comamonas testosteroni CNB-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CTCNB1_RS15470
• Protein ID: WP_012838959.1
• Coordinates: 3411353..3411649 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CTCNB1_RS15465
• Protein ID: WP_012838958.1
• Coordinates: 3411054..3411350 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTCNB1_RS15420	3406464..3406742	-	279	WP_012838951.1	hypothetical protein	-
CTCNB1_RS15425	3406729..3407646	-	918	WP_012838952.1	toprim domain-containing protein	-
CTCNB1_RS15430	3407643..3407891	-	249	WP_012838953.1	hypothetical protein	-
CTCNB1_RS15435	3407888..3408103	-	216	WP_123975013.1	hypothetical protein	-
CTCNB1_RS15440	3408115..3408477	-	363	WP_123975015.1	hypothetical protein	-
CTCNB1_RS15445	3408481..3408738	-	258	WP_012838955.1	hypothetical protein	-
CTCNB1_RS15450	3408744..3408947	-	204	WP_012838956.1	hypothetical protein	-
CTCNB1_RS15455	3409250..3409594	-	345	WP_012838957.1	hypothetical protein	-
CTCNB1_RS25400	3409946..3410923	-	978	WP_123975016.1	hypothetical protein	-
CTCNB1_RS15465	3411054..3411350	-	297	WP_012838958.1	putative addiction module antidote protein	Antitoxin
CTCNB1_RS15470	3411353..3411649	-	297	WP_012838959.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CTCNB1_RS15475	3411909..3413258	-	1350	WP_012838960.1	integrase family protein	-
CTCNB1_RS15480	3413818..3414036	+	219	WP_003053604.1	hypothetical protein	-
CTCNB1_RS15485	3414060..3414323	+	264	WP_003061434.1	translation initiation factor IF-1	-
CTCNB1_RS15495	3415010..3416578	-	1569	WP_012838962.1	sensor domain-containing diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3396786..3414323	17537

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (9-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11023.67 Da
• Isoelectric Point: 7.2027

>T24402 WP_012838959.1 NC_013446:c3411649-3411353 [Comamonas thiooxydans]
MFTIKRTAEFDAWLFGLRDTMTRIRLAKRLDKVQRGLLGDTKPVGDGVHELREDFGPGWRMYYVQHGDVLVVMLGGGDKS
TQQADIQAAIALSKTLED

Nucleotide

• Length: 297 bp

>T24402 NC_013446:c3411649-3411353 [Comamonas thiooxydans]
ATGTTCACCATCAAGCGAACCGCTGAATTTGACGCCTGGTTATTCGGCTTGCGCGACACCATGACACGCATCCGTCTGGC
CAAGAGGCTGGACAAGGTGCAGCGCGGCCTGCTTGGAGATACCAAACCCGTGGGCGATGGAGTGCACGAGCTGCGCGAGG
ACTTCGGCCCCGGCTGGCGCATGTACTACGTGCAGCACGGCGATGTGTTGGTGGTGATGCTGGGTGGTGGGGACAAGTCC
ACCCAACAGGCCGATATTCAGGCCGCGATTGCTTTGTCCAAGACACTGGAGGACTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10575.14 Da
• Isoelectric Point: 5.1006

>AT24402 WP_012838958.1 NC_013446:c3411350-3411054 [Comamonas thiooxydans]
MTKIADLPTFDMAEHLKNDEDVAAYLSLVLEEDDPAELANALGVIARARGMTEIASASGLTREALYKALRPGSQPRFDTI
RRVCGALGVRLVAQPIHA

Nucleotide

• Length: 297 bp

>AT24402 NC_013446:c3411350-3411054 [Comamonas thiooxydans]
ATGACCAAAATTGCAGACCTGCCCACCTTTGATATGGCAGAGCACTTGAAAAACGATGAGGACGTGGCTGCCTATCTGTC
GCTGGTGCTGGAAGAAGATGACCCCGCAGAGTTGGCCAACGCCCTTGGCGTGATTGCTCGTGCCCGCGGTATGACTGAGA
TTGCCAGCGCCAGCGGCCTGACCCGTGAAGCGCTGTACAAGGCACTTCGCCCAGGCAGCCAGCCTCGCTTCGACACCATC
CGCCGTGTCTGTGGTGCTCTGGGCGTGCGCCTAGTGGCCCAGCCTATCCATGCCTGA