Detailed information of TA system TA024398

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1763109..1763589
• Replicon: chromosome
• Accession: NC_013422
• Organism: Halothiobacillus neapolitanus c2

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: D0L180
• Locus tag: HNEAP_RS08120
• Protein ID: WP_012824487.1
• Coordinates: 1763109..1763381 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: D0L181
• Locus tag: HNEAP_RS08125
• Protein ID: WP_012824488.1
• Coordinates: 1763365..1763589 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HNEAP_RS08090	1758340..1758636	-	297	WP_012824480.1	VirB3 family type IV secretion system protein	-
HNEAP_RS08095	1758692..1759111	-	420	WP_012824481.1	TrbC/VirB2 family protein	-
HNEAP_RS08100	1759192..1759692	-	501	WP_012824482.1	GNAT family N-acetyltransferase	-
HNEAP_RS08105	1759689..1759970	-	282	WP_012824483.1	DUF1778 domain-containing protein	-
HNEAP_RS08110	1760053..1761093	-	1041	WP_012824484.1	Flp pilus assembly complex ATPase component TadA	-
HNEAP_RS08115	1761166..1762590	-	1425	WP_012824485.1	hypothetical protein	-
HNEAP_RS08120	1763109..1763381	-	273	WP_012824487.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HNEAP_RS08125	1763365..1763589	-	225	WP_012824488.1	TraY domain-containing protein	Antitoxin
HNEAP_RS08130	1763644..1764129	-	486	WP_049772508.1	helix-turn-helix domain-containing protein	-
HNEAP_RS08135	1764128..1764838	+	711	WP_012824490.1	hypothetical protein	-
HNEAP_RS12290	1764943..1766412	+	1470	WP_012824491.1	hypothetical protein	-
HNEAP_RS08145	1766800..1768263	+	1464	WP_012824492.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-88)

Antitoxin

• Relaxosome protein TraY (4-49)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10425.09 Da
• Isoelectric Point: 10.2886

>T24398 WP_012824487.1 NC_013422:c1763381-1763109 [Halothiobacillus neapolitanus c2]
MAWQIEFDDASKKDLAKLDKQIARRITEFLRERVTVLDDPRSIGEALKGSKLGAFWKYRVGDYRIIASIEDGALRILVVK
IGNRKDVYRQ

Nucleotide

• Length: 273 bp

>T24398 NC_013422:c1763381-1763109 [Halothiobacillus neapolitanus c2]
TTGGCTTGGCAGATTGAATTCGACGATGCTTCCAAGAAGGACTTGGCAAAGTTGGACAAGCAGATCGCTCGACGCATCAC
GGAATTTTTGCGCGAGCGTGTGACAGTACTGGACGACCCGCGCAGCATTGGCGAAGCCCTCAAAGGTTCCAAGTTGGGGG
CTTTTTGGAAATACCGCGTAGGCGACTATCGCATCATCGCCAGCATCGAGGACGGCGCCTTGCGCATCCTTGTTGTGAAG
ATTGGCAACCGCAAGGATGTTTACCGCCAATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8418.57 Da
• Isoelectric Point: 4.6412

>AT24398 WP_012824488.1 NC_013422:c1763589-1763365 [Halothiobacillus neapolitanus c2]
MLAIRLPAEVENRLDALAKATGRTKTFYAREAILEHLDDLEDLYLAEQRLIDIRAGRSKTYSLEEVERSLGLAD

Nucleotide

• Length: 225 bp

>AT24398 NC_013422:c1763589-1763365 [Halothiobacillus neapolitanus c2]
ATGCTTGCCATCCGACTACCCGCCGAAGTGGAAAACCGTCTTGATGCACTGGCAAAGGCCACTGGGCGTACCAAAACTTT
CTATGCCCGCGAAGCCATCCTTGAGCACTTGGATGACCTCGAAGATTTGTACCTTGCAGAGCAACGCCTGATCGACATTC
GCGCAGGCCGCAGCAAGACCTACAGCTTGGAAGAAGTGGAGCGCAGCCTTGGCTTGGCAGATTGA

Structures

Toxin

• AlphaFold DB: D0L180

Antitoxin

• AlphaFold DB: D0L181