Detailed information of TA system TA024397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 296954..297495
• Replicon: chromosome
• Accession: NC_013422
• Organism: Halothiobacillus neapolitanus c2

Toxin (Protein)

• Gene name: parE
• Uniprot ID: D0KXD5
• Locus tag: HNEAP_RS01390
• Protein ID: WP_012823185.1
• Coordinates: 296954..297256 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: D0KXD6
• Locus tag: HNEAP_RS01395
• Protein ID: WP_012823186.1
• Coordinates: 297256..297495 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HNEAP_RS01380	294221..295990	+	1770	WP_012823183.1	hypothetical protein	-
HNEAP_RS01385	296366..296713	+	348	WP_012823184.1	DUF3024 domain-containing protein	-
HNEAP_RS01390	296954..297256	-	303	WP_012823185.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HNEAP_RS01395	297256..297495	-	240	WP_012823186.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
HNEAP_RS01400	297650..298324	-	675	WP_012823187.1	anti-sigma factor	-
HNEAP_RS01405	298326..298949	-	624	WP_012823188.1	sigma-70 family RNA polymerase sigma factor	-
HNEAP_RS12780	298942..299349	+	408	WP_041600324.1	hypothetical protein	-
HNEAP_RS01415	299368..299625	+	258	WP_012823189.1	hypothetical protein	-
HNEAP_RS01420	299957..300805	+	849	WP_012823190.1	DUF692 domain-containing protein	-
HNEAP_RS12120	300802..301551	+	750	WP_012823191.1	putative DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Antitoxin ParD (1-77)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11481.22 Da
• Isoelectric Point: 8.5096

>T24397 WP_012823185.1 NC_013422:c297256-296954 [Halothiobacillus neapolitanus c2]
MGKFSLSHSAKADLRGIARFTEKHWGRAQRLHYLKGLDETFKILADSPTLGNACDYIETGVRKYPFQSHVVFYECLSNEE
ILVVRMLHKNMDVHQAVFPA

Nucleotide

• Length: 303 bp

>T24397 NC_013422:c297256-296954 [Halothiobacillus neapolitanus c2]
ATGGGCAAATTTTCTCTTAGCCACTCCGCAAAAGCCGATTTGCGTGGAATCGCACGATTCACAGAAAAACATTGGGGGAG
AGCACAACGCCTGCATTACCTCAAAGGCTTGGATGAAACTTTCAAGATACTGGCCGATTCGCCAACACTTGGTAATGCCT
GTGATTACATCGAAACAGGTGTGCGCAAATATCCTTTTCAGAGCCATGTGGTATTTTACGAGTGCCTCTCAAACGAGGAG
ATTCTAGTGGTGCGCATGCTTCATAAAAATATGGATGTTCATCAGGCTGTTTTCCCCGCCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8810.83 Da
• Isoelectric Point: 4.7806

>AT24397 WP_012823186.1 NC_013422:c297495-297256 [Halothiobacillus neapolitanus c2]
MAKNTSVSLGNHFDQFIAAQLSKGRYGSATEVVRAGLRLLENEEQKLETLRQLIAEGRASGTAEYNYEKFMAELDNELS

Nucleotide

• Length: 240 bp

>AT24397 NC_013422:c297495-297256 [Halothiobacillus neapolitanus c2]
ATGGCAAAAAACACCAGTGTCAGCTTGGGAAACCATTTTGATCAGTTTATTGCAGCTCAACTGTCGAAAGGCCGCTATGG
CTCAGCGACAGAGGTGGTGCGCGCTGGTTTGCGCCTGCTTGAGAATGAAGAACAGAAACTGGAAACACTGCGCCAATTAA
TAGCAGAAGGCCGAGCCAGTGGAACGGCTGAATACAACTATGAAAAGTTTATGGCCGAACTCGATAATGAATTGAGTTAA

Structures

Toxin

• AlphaFold DB: D0KXD5

Antitoxin

• AlphaFold DB: D0KXD6