Detailed information of TA system TA024395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 252850..253417
• Replicon: chromosome
• Accession: NC_013422
• Organism: Halothiobacillus neapolitanus c2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D0KX93
• Locus tag: HNEAP_RS01185
• Protein ID: WP_012823143.1
• Coordinates: 252850..253179 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D0KX94
• Locus tag: HNEAP_RS01190
• Protein ID: WP_012823144.1
• Coordinates: 253166..253417 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HNEAP_RS12105	248330..249076	+	747	WP_012823136.1	class I SAM-dependent methyltransferase	-
HNEAP_RS01165	249073..249999	+	927	WP_012823137.1	hypothetical protein	-
HNEAP_RS12430	249996..250550	+	555	WP_012823138.1	acyltransferase	-
HNEAP_RS01170	250516..251679	-	1164	WP_012823139.1	DegT/DnrJ/EryC1/StrS family aminotransferase	-
HNEAP_RS01175	251829..252146	-	318	WP_041600537.1	hypothetical protein	-
HNEAP_RS01180	252310..252606	-	297	WP_012823141.1	nucleotidyltransferase domain-containing protein	-
HNEAP_RS01185	252850..253179	-	330	WP_012823143.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HNEAP_RS01190	253166..253417	-	252	WP_012823144.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
HNEAP_RS01195	253598..253858	-	261	WP_012823145.1	Txe/YoeB family addiction module toxin	-
HNEAP_RS01200	253855..254106	-	252	WP_012823146.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
HNEAP_RS01205	254253..254816	-	564	WP_012823147.1	Uma2 family endonuclease	-
HNEAP_RS12625	254854..255018	-	165	WP_012823148.1	hypothetical protein	-
HNEAP_RS01210	255234..256067	-	834	WP_012823149.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-
HNEAP_RS01215	256158..257315	-	1158	WP_012823150.1	carbohydrate porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (15-94)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-77)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12421.42 Da
• Isoelectric Point: 9.8939

>T24395 WP_012823143.1 NC_013422:c253179-252850 [Halothiobacillus neapolitanus c2]
MTKSDASPSATRYRLKFLPEALDEWNALDGSIKELLRKLLKKRLNEPHTPGAQLHGDLRGCYKIKLRKQGYRLVYSVEDN
VLVVLVLAIDKREDMAAYHSALDRLISGQ

Nucleotide

• Length: 330 bp

>T24395 NC_013422:c253179-252850 [Halothiobacillus neapolitanus c2]
TTGACCAAATCTGATGCAAGCCCAAGTGCGACACGCTACCGGCTAAAATTCCTACCGGAAGCCCTTGACGAATGGAACGC
ACTCGACGGCAGCATCAAAGAACTATTACGCAAATTACTCAAAAAACGCCTCAACGAACCGCATACGCCGGGTGCGCAAC
TGCATGGTGATTTGCGCGGGTGCTACAAAATCAAACTACGCAAACAGGGCTATCGCCTTGTGTACAGCGTCGAAGATAAT
GTGCTGGTCGTACTCGTGCTCGCCATCGACAAACGCGAAGACATGGCCGCTTATCATTCGGCTCTTGATCGCCTGATATC
TGGTCAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9478.93 Da
• Isoelectric Point: 5.6863

>AT24395 WP_012823144.1 NC_013422:c253417-253166 [Halothiobacillus neapolitanus c2]
MDAIYADYSISMTEFKKNPAHVLRTAGEKPVAVLNHNRPAFYMVSPKLFEAIAEELSDKNLLELARQRLMRKDSAIEVDI
DQI

Nucleotide

• Length: 252 bp

>AT24395 NC_013422:c253417-253166 [Halothiobacillus neapolitanus c2]
ATGGACGCTATCTACGCCGACTACTCCATCAGCATGACCGAATTCAAAAAGAACCCGGCCCATGTCTTGCGAACGGCAGG
CGAAAAGCCGGTCGCCGTGCTCAACCACAACCGGCCCGCGTTCTACATGGTTAGCCCCAAACTCTTTGAAGCCATCGCCG
AAGAGCTATCCGACAAAAACCTGCTCGAATTGGCACGTCAGCGTTTAATGCGCAAAGACAGCGCTATTGAGGTGGATATT
GACCAAATCTGA

Structures

Toxin

• AlphaFold DB: D0KX93

Antitoxin

• AlphaFold DB: D0KX94