Detailed information of TA system TA024393

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 1503093..1503818
• Replicon: chromosome
• Accession: NC_013421
• Organism: Pectobacterium wasabiae WPP163

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: PECWA_RS06815
• Protein ID: WP_015729923.1
• Coordinates: 1503093..1503413 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: PECWA_RS06820
• Protein ID: WP_015729924.1
• Coordinates: 1503483..1503818 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PECWA_RS06795	1498287..1498577	-	291	WP_015729918.1	transposase	-
PECWA_RS23170	1498643..1498972	+	330	Protein_1349	IS30 family transposase	-
PECWA_RS06800	1499146..1499784	-	639	WP_015729919.1	hypothetical protein	-
PECWA_RS06805	1500215..1501429	-	1215	WP_015729920.1	tyrosine-type recombinase/integrase	-
PECWA_RS24070	1501790..1501960	-	171	WP_157681915.1	hypothetical protein	-
PECWA_RS06810	1502142..1502975	-	834	WP_015729922.1	DUF4942 domain-containing protein	-
PECWA_RS06815	1503093..1503413	-	321	WP_015729923.1	TA system toxin CbtA family protein	Toxin
PECWA_RS06820	1503483..1503818	-	336	WP_015729924.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
PECWA_RS06825	1503858..1504331	-	474	WP_015729925.1	DNA repair protein RadC	-
PECWA_RS06830	1504416..1504649	-	234	WP_015729926.1	membrane protein	-
PECWA_RS06835	1504667..1505140	-	474	WP_015729927.1	hypothetical protein	-
PECWA_RS06840	1505176..1505745	-	570	WP_015729928.1	hypothetical protein	-
PECWA_RS06845	1505927..1506625	-	699	WP_015729929.1	DeoR family transcriptional regulator	-
PECWA_RS06850	1506995..1507870	-	876	WP_015729930.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1496023..1518405	22382

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-105)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12064.83 Da
• Isoelectric Point: 4.5514

>T24393 WP_015729923.1 NC_013421:c1503413-1503093 [Pectobacterium parmentieri WPP163]
MQTLPGIPPREDNPCPSPITVWQQLLTYLLEQHYGLMLNDTPFCEENVIQAHIDAGVTLVNAVNFLVEKYELVRIDRDGF
NWQEQSPFLTAVDVLRARRATGLLKA

Nucleotide

• Length: 321 bp

>T24393 NC_013421:c1503413-1503093 [Pectobacterium parmentieri WPP163]
ATGCAAACATTACCCGGAATCCCTCCACGGGAGGATAACCCCTGTCCGTCACCTATAACCGTCTGGCAACAACTACTGAC
CTACCTGCTGGAACAGCACTACGGCCTGATGCTGAACGATACACCATTCTGTGAGGAGAACGTGATTCAGGCGCATATCG
ATGCTGGCGTCACGTTGGTCAATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGATCGCGACGGTTTT
AACTGGCAGGAGCAATCGCCGTTTCTCACTGCCGTTGATGTTCTCCGTGCCAGACGTGCTACCGGCTTGCTCAAGGCATA
A

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12441.16 Da
• Isoelectric Point: 6.0660

>AT24393 WP_015729924.1 NC_013421:c1503818-1503483 [Pectobacterium parmentieri WPP163]
MPSSDTPEWGLKCAITPRFGARLVQEGHRLHYLADRASVVGTFSKTEAQSLERCFPKLIELLEQKLRMEELNPHQQGCIT
LHCNEWTCEADTLGSVGYVYIAIYPSSAATQ

Nucleotide

• Length: 336 bp

>AT24393 NC_013421:c1503818-1503483 [Pectobacterium parmentieri WPP163]
ATGCCATCATCGGATACCCCTGAATGGGGACTAAAATGTGCCATCACTCCGCGATTCGGAGCCAGGCTGGTGCAGGAAGG
TCACCGTCTGCACTATCTGGCAGACCGGGCCAGCGTTGTCGGTACGTTTAGCAAAACAGAAGCCCAAAGTCTGGAACGTT
GCTTCCCTAAGCTAATTGAGCTGTTGGAACAGAAGCTACGCATGGAGGAACTGAATCCCCACCAGCAGGGCTGCATCACG
CTGCATTGCAACGAGTGGACCTGTGAAGCCGATACGCTGGGCAGTGTTGGCTACGTGTATATTGCTATTTATCCCTCTTC
AGCGGCAACACAATAA